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Validation Data Gallery

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  • Western Blot (WB) analysis of rat kidney tissue using SLC3A1 Recombinant antibody (84856-2-RR)

    WB analysis of rat kidney using 84856-2-RR

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 84856-2-RR (SLC3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using SLC3A1 Recombinant antibody (84856-2-RR)

    WB analysis of mouse kidney using 84856-2-RR

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 84856-2-RR (SLC3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84856-2-RR

    Affinity and Kinetic Characterization of 84856-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84856-2-RR against Human SLC3A1 were performed. The affinity constant is below 1 pM.

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SLC3A1 Recombinant antibody

SLC3A1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84856-2-RR
Clone No.242370D3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

D2H, CSNU1, b(0,+)-type amino acid transporter-related heavy chain, ATR1, Amino acid transporter heavy chain SLC3A1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat kidney tissue, mouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84856-2-RR targets SLC3A1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SLC3A1 fusion protein Ag9525 相同性解析による交差性が予測される生物種
Full Name solute carrier family 3 (cystine, dibasic and neutral amino acid transporters, activator of cystine, dibasic and neutral amino acid transport), member 1
Calculated molecular weight 685 aa, 79 kDa
Observed molecular weight78-90 kDa
GenBank accession numberBC022386
Gene Symbol SLC3A1
Gene ID (NCBI) 6519
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ07837
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC3A1(Neutral and basic amino acid transport protein rBAT) is also named as NBAT,D2h,RBAT. It is involved in the high-affinity, sodium-independent transport of cystine and neutral and dibasic amino acids (system B(0,+)-like activity).SLC3A1 can be degraded, at least in part, via the endoplasmic reticulum-associated degradation (ERAD) pathway(PMID:18332091). SLC3A1 has different isoforms vary from 79 kDa to 36kDa and the 85 kDa glycosylated protein can be detected in SDS-PAGE. (PMID:11546643)

Protocols

Product Specific Protocols
WB protocol for SLC3A1 antibody 84856-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols