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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC43A1 Polyclonal antibody

SLC43A1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10444-1-AP

Synonyms

LAT3, PB39, POV1, R00504, SLC43A1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC43A1 Polyclonal antibody (10444-1-AP)

    WB analysis using 10444-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 10444-1-AP (SLC43A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SLC43A1 Polyclonal antibody (10444-1-AP)

    WB analysis using 10444-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10444-1-AP (SLC43A1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SLC43A1 Polyclonal antibody (10444-1-AP)

    IHC staining of human kidney using 10444-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10444-1-AP (SLC43A1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SLC43A1 Polyclonal antibody (10444-1-AP)

    IHC staining of human kidney using 10444-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10444-1-AP (SLC43A1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inPC-3 cells, Daudi cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10444-1-AP targets SLC43A1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC43A1 fusion protein Ag0305 相同性解析による交差性が予測される生物種
Full Name solute carrier family 43, member 1
Calculated molecular weight 61 kDa
Observed molecular weight55-61 kDa
GenBank accession numberBC001639
Gene symbol SLC43A1
Gene ID (NCBI) 8501
RRIDAB_2302076
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC43A1, also named as LAT3, PB39 and POV1, belongs to the system L family of plasma membrane carrier proteins that transports large neutral amino acids. It plays a role in the development of human prostate cancer, from prostatic intraepithelial neoplasia to invasive prostate cancer.

Protocols

Product Specific Protocols
WB protocol for SLC43A1 antibody 10444-1-APDownload protocol
IHC protocol for SLC43A1 antibody 10444-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Arch Biochem Biophys

Enhancement of chemoresistance by claudin-1-mediated formation of amino acid barriers in human lung adenocarcinoma A549 cells

Authors - Riho Kimura
View Article