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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC43A3 Polyclonal antibody

SLC43A3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20131-1-AP

Synonyms

Protein FOAP 13, PRO1659, FOAP 13, Equilibrative nucleobase transporter 1, ENBT1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC43A3 Polyclonal antibody (20131-1-AP)

    WB analysis using 20131-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20131-1-AP (SLC43A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using SLC43A3 Polyclonal antibody (20131-1-AP)

    IHC staining of human ovary cancer using 20131-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20131-1-AP (SLC43A3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using SLC43A3 Polyclonal antibody (20131-1-AP)

    IHC staining of human ovary cancer using 20131-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20131-1-AP (SLC43A3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHL-60 cells, MCF-7 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20131-1-AP targets SLC43A3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC43A3 fusion protein Ag13999 相同性解析による交差性が予測される生物種
Full Name solute carrier family 43, member 3
Calculated molecular weight 491 aa, 55 kDa
Observed molecular weight55 kDa
GenBank accession numberBC003163
Gene symbol SLC43A3
Gene ID (NCBI) 29015
RRIDAB_3085599
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sodium-independent purine-selective nucleobase transporter(SLC43A3), also called as equilibrative nucleobase transporter 1(ENBT1), is a nucleobase transporter involved in purine salvage pathways in mammals, which mediates the equilibrative transport of extracellular purine nucleobases such as adenine, guanine and hypoxanthine.(PubMed:26455426, PubMed:32339528) It has been found that it may regulate the transport of fatty acids (FA) in fat cells, as a positive regulator of FA efflux and as a negative regulator of FA uptake.(PMID: 32217606)

Protocols

Product Specific Protocols
WB protocol for SLC43A3 antibody 20131-1-APDownload protocol
IHC protocol for SLC43A3 antibody 20131-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols