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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Band 3/AE 1 Recombinant antibody

Band 3/AE 1 Recombinant Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3K21

Cat no : 81066-1-RR

Synonyms

Band 3 / AE1, band 3, band 3/ AE1, SLC4A1, Anion exchanger 1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human placenta tissue using Band 3/ AE1 Recombinant antibody (81066-1-RR)

    IHC staining of human placenta using 81066-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81066-1-RR (SLC4A1 antibody) at dilution of 1:1700 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Band 3/ AE1 Recombinant antibody (81066-1-RR)

    IHC staining of human placenta using 81066-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81066-1-RR (SLC4A1 antibody) at dilution of 1:1700 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using Band 3/AE 1 Recombinant antibody (81066-1-RR)

    IF Staining of human placenta using 81066-1-RR

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Band 3/ AE1 antibody (81066-1-RR, Clone: 3K21 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using Band 3/AE 1 Recombinant antibody (81066-1-RR)

    IF Staining of human placenta using 81066-1-RR

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Band 3/ AE1 antibody (81066-1-RR, Clone: 3K21 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 81066-1-RR

    Affinity and Kinetic Characterization of 81066-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81066-1-RR against Human Band 3/AE 1 were performed. The affinity constant is 23.6 pM.

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Tested Applications

Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81066-1-RR targets Band 3/AE 1 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Band 3/AE 1 fusion protein Ag27878 相同性解析による交差性が予測される生物種
Full Name solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
Calculated molecular weight 911 aa, 102 kDa
GenBank accession numberBC101570
Gene symbol band 3
Gene ID (NCBI) 6521
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Anion exchanger 1 (AE1), also known as band 3, is a 95-100 kDa transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3-. It contains a 52-55 kDa C-terminal membrane crossing domain and a 43 kDa N-terminal cytoplasmic domain. Degradation of band 3 protein occurred during erythrocyte aging. The oxidative stress can also activate the proteolysis of band 3 through caspases.

Protocols

Product Specific Protocols
IHC protocol for Band 3/AE 1 antibody 81066-1-RRDownload protocol
IF protocol for Band 3/AE 1 antibody 81066-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols