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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC5A11 Polyclonal antibody (14089-1-AP)

    WB analysis using 14089-1-AP

    C2C12 cells were subjected to SDS PAGE followed by western blot with 14089-1-AP (SLC5A11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SLC5A11 Polyclonal antibody (14089-1-AP)

    IHC staining of human colon cancer using 14089-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14089-1-AP (SLC5A11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SLC5A11 Polyclonal antibody (14089-1-AP)

    IHC staining of human colon cancer using 14089-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14089-1-AP (SLC5A11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using SLC5A11 Polyclonal antibody (14089-1-AP)

    IHC staining of mouse skeletal muscle using 14089-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14089-1-AP (SLC5A11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using SLC5A11 Polyclonal antibody (14089-1-AP)

    IHC staining of mouse skeletal muscle using 14089-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14089-1-AP (SLC5A11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SLC5A11 Polyclonal antibody

SLC5A11 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14089-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

KST1, RKST1, SGLT6, SLC5A11, SLGTX, SMIT2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inC2C12 cells
Positive IHC detected inhuman colon cancer tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

14089-1-AP targets SLC5A11 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC5A11 fusion protein Ag5220 相同性解析による交差性が予測される生物種
Full Name solute carrier family 5 (sodium/glucose cotransporter), member 11
Calculated molecular weight 611 aa, 67 kDa
Observed molecular weight 74 kDa
GenBank accession numberBC057780
Gene Symbol SLC5A11
Gene ID (NCBI) 115584
RRIDAB_2189540
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WWX8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SLC5A11 antibody 14089-1-APDownload protocol
IHC protocol for SLC5A11 antibody 14089-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Clin Endocrinol Metab

Placental Inositol Reduced in Gestational Diabetes as Glucose Alters Inositol Transporters and IMPA1 Enzyme Expression.

Authors - Reshma A Pillai
View Article
humanWB

Front Pharmacol

Quercetin Attenuates Atherosclerosis via Modulating Oxidized LDL-Induced Endothelial Cellular Senescence.

Authors - Yue-Hua Jiang
View Article