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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC5A6 Polyclonal antibody

SLC5A6 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 26407-1-AP

Synonyms

SLC5A6, SMVT

Validation Data Gallery

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  • Western Blot (WB) analysis of Caco-2 cells using SLC5A6 Polyclonal antibody (26407-1-AP)

    WB analysis of Caco-2 using 26407-1-AP

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SLC5A6 Polyclonal antibody (26407-1-AP)

    WB analysis of mouse brain using 26407-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse small intestine tissue using SLC5A6 Polyclonal antibody (26407-1-AP)

    WB analysis of mouse small intestine using 26407-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using SLC5A6 Polyclonal antibody (26407-1-AP)

    WB analysis of COLO 320 using 26407-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of COLO 320 cells using SLC5A6 Polyclonal antibody (26407-1-AP)

    WB analysis of COLO 320 using 26407-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using SLC5A6 Polyclonal antibody (26407-1-AP)

    WB analysis of rat brain using 26407-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SLC5A6 Polyclonal antibody (26407-1-AP)

    IP experiment of mouse brain using 26407-1-AP

    IP result of anti-SLC5A6 (IP:26407-1-AP, 4ug; Detection:26407-1-AP 1:500) with mouse brain tissue lysate 5200ug.

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Tested Applications

Positive WB detected inCaco-2 cells, mouse brain tissue, mouse small intestine tissue, COLO 320 cells, rat brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 26407-1-AP is SLC5A6 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC5A6 fusion protein Ag23907 相同性解析による交差性が予測される生物種
Full Name solute carrier family 5 (sodium-dependent vitamin transporter), member 6
Observed molecular weight 69 kDa
GenBank accession numberBC012806
Gene symbol SLC5A6
Gene ID (NCBI) 8884
RRIDAB_2880502
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SLC5A6 antibody 26407-1-APDownload protocol
IP protocol for SLC5A6 antibody 26407-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Nat Metab

Vitamin B5 supports MYC oncogenic metabolism and tumor progression in breast cancer

Authors - Peter Kreuzaler
View Article
humanWB

Mol Cell Proteomics

Hypoxia is a dominant remodeler of the effector T cell surface proteome relative to activation and regulatory T cell suppression.

Authors - James R Byrnes
View Article