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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC5A8 Polyclonal antibody (29827-1-AP)

    WB analysis using 29827-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29827-1-AP (SLC5A8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat brain tissue using SLC5A8 Polyclonal antibody (29827-1-AP)

    IHC staining of rat brain using 29827-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 29827-1-AP (SLC5A8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using SLC5A8 Polyclonal antibody (29827-1-AP)

    IHC staining of rat brain using 29827-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 29827-1-AP (SLC5A8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SLC5A8 Polyclonal antibody

SLC5A8 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29827-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Sodium-coupled monocarboxylate transporter 1, Sodium iodide-related cotransporter, SMCT1, SMCT, Apical iodide transporter

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29827-1-AP targets SLC5A8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC5A8 fusion protein Ag30183 相同性解析による交差性が予測される生物種
Full Name solute carrier family 5 (iodide transporter), member 8
Calculated molecular weight 610 aa, 67 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC110492
Gene Symbol SLC5A8
Gene ID (NCBI) 160728
RRIDAB_3086176
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N695
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The sodium-coupled monocarboxylate transporter 1 (SMCT1; SLC5A8), belonging to the SLC5A family, is a 67-kDa protein that functions as a Na+-coupled electrogenic transporter for SCFAs. SLC5A mRNA and protein are expressed in healthy human, rat, and mouse colon. The protein is localized to the apical membrane of the colonocytes. Immunohistochemical studies revealed the selective localization of SMCT1 on the brush border in the villi of the terminal ileum and crypts of the colon in mice.

Protocols

Product Specific Protocols
WB protocol for SLC5A8 antibody 29827-1-APDownload protocol
IHC protocol for SLC5A8 antibody 29827-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols