SLC6A12 Monoclonal antibody
SLC6A12 Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Mouse, Rat and More (2)
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
2C1G4
Cat no : 67700-1-Ig
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | L02 cells, rat liver tissue, HSC-T6 cells, HuH-7 cells, HepG2 cells, mouse hepatocytes |
| Positive IF/ICC detected in | Caco-2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
67700-1-Ig targets SLC6A12 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | mouse, canine |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SLC6A12 fusion protein Ag18962 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12 |
| Calculated molecular weight | 614 aa, 69 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC126215 |
| Gene symbol | SLC6A12 |
| Gene ID (NCBI) | 6539 |
| RRID | AB_2882892 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC6A12 antibody 67700-1-Ig | Download protocol |
| IF protocol for SLC6A12 antibody 67700-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Am J Physiol Renal Physiol Mutation of a single threonine in the cytoplasmic NH2 terminus disrupts trafficking of renal betaine-GABA transporter 1 during hypertonic stress. | ||
Int J Biol Sci Sodium chloride promotes macrophage pyroptosis and aggravates rheumatoid arthritis by activating SGK1 through GABA receptors Slc6a12 |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Hua (Verified Customer) (06-17-2021) | It works ok. The signal is very weak at 1:2000 dilution. The diluted antibody solution can only be used once.
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