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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC6A12 Monoclonal antibody (67700-1-Ig)

    WB analysis using 67700-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67700-1-Ig (SLC6A12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SLC6A12 Monoclonal antibody (67700-1-Ig)

    WB analysis using 67700-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67700-1-Ig (SLC6A12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using SLC6A12 Monoclonal antibody (67700-1-Ig)

    WB analysis of HSC-T6 using 67700-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67700-1-Ig (SLC6A12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using SLC6A12 Monoclonal antibody (67700-1-Ig)

    IF Staining of Caco-2 using 67700-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed Caco-2 cells using SLC6A12 antibody (67700-1-Ig, Clone: 2C1G4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using SLC6A12 Monoclonal antibody (67700-1-Ig)

    IF Staining of Caco-2 using 67700-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using SLC6A12 antibody (67700-1-Ig, Clone: 2C1G4 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L).

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SLC6A12 Monoclonal antibody

SLC6A12 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67700-1-Ig
Clone No.2C1G4
Publications(2)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IF/ICC, ELISA

Synonyms

BGT 1, BGT1, FLJ38727, SLC6A12

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inL02 cells, rat liver tissue, HSC-T6 cells, HuH-7 cells, HepG2 cells, mouse hepatocytes
Positive IF/ICC detected inCaco-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

67700-1-Ig targets SLC6A12 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivity mouse, canine
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SLC6A12 fusion protein Ag18962 相同性解析による交差性が予測される生物種
Full Name solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12
Calculated molecular weight 614 aa, 69 kDa
Observed molecular weight70 kDa
GenBank accession numberBC126215
Gene Symbol SLC6A12
Gene ID (NCBI) 6539
RRIDAB_2882892
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP48065
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SLC6A12 antibody 67700-1-IgDownload protocol
IF protocol for SLC6A12 antibody 67700-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
canineWB

Am J Physiol Renal Physiol

Mutation of a single threonine in the cytoplasmic NH2 terminus disrupts trafficking of renal betaine-GABA transporter 1 during hypertonic stress.

Authors - Eva S Schweikhard
View Article
mouseWB

Int J Biol Sci

Sodium chloride promotes macrophage pyroptosis and aggravates rheumatoid arthritis by activating SGK1 through GABA receptors Slc6a12

Authors - Xianzheng Zhang
View Article