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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC6A19 Polyclonal antibody

SLC6A19 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse , rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29372-1-AP

Synonyms

B0AT1, FLJ20680, FLJ34635, HND, SLC6A19

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC6A19 Polyclonal antibody (29372-1-AP)

    WB analysis using 29372-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29372-1-AP (SLC6A19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SLC6A19 Polyclonal antibody (29372-1-AP)

    WB analysis using 29372-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29372-1-AP (SLC6A19 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SLC6A19 Polyclonal antibody (29372-1-AP)

    IHC staining of human kidney using 29372-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29372-1-AP (SLC6A19 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SLC6A19 Polyclonal antibody (29372-1-AP)

    IHC staining of human kidney using 29372-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29372-1-AP (SLC6A19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using SLC6A19 Polyclonal antibody (29372-1-AP)

    IHC staining of human small intestine using 29372-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 29372-1-AP (SLC6A19 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using SLC6A19 Polyclonal antibody (29372-1-AP)

    IHC staining of human small intestine using 29372-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 29372-1-AP (SLC6A19 antibody) at dilution of 1:200 (under 10x lens).

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Tested Applications

Positive WB detected inmouse kidney tissue, HeLa cells, rat kidney
Positive IHC detected inhuman kidney tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

29372-1-AP targets SLC6A19 in WB, IHC, ELISA applications and shows reactivity with Human, mouse , rat samples.

Tested Reactivity Human, mouse , rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC6A19 fusion protein Ag31076 相同性解析による交差性が予測される生物種
Full Name solute carrier family 6 (neutral amino acid transporter), member 19
Calculated molecular weight 634 aa, 71 kDa
Observed molecular weight71 kDa
GenBank accession numberBC148801
Gene symbol SLC6A19
Gene ID (NCBI) 340024
RRIDAB_2918286
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC6A19, also named as B0AT1, encodes system B(0) transmembrane protein that mediates epithelial resorption of neutral amino acids across the apical membrane in kidney and intestine (PubMed: 15044460). The protein contains 634 amino acids and has 12 predicted transmembrane regions with highly expression in human kidney and small intestine and minimal in pancreas (PubMed: 15286787). The expression of SLC6A19 in intestinal cells depends on angiotensin converting enzyme 2 which is the cellular receptor for SARS-CoV-2 (PubMed: 32132184). Mutations in SLC6A19 cause Hartnup disease (PMID: 15286787, PMID: 15286788).

Protocols

Product Specific Protocols
WB protocol for SLC6A19 antibody 29372-1-APDownload protocol
IHC protocol for SLC6A19 antibody 29372-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

J Ethnopharmacol

Metabolomics and molecular docking-directed anti-obesity study of the ethanol extract from Gynostemma pentaphyllum (Thunb.) Makino

Authors - Suyun Xu
View Article