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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC6A20 Polyclonal antibody (30843-1-AP)

    WB analysis using 30843-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30843-1-AP (SLC6A20 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SLC6A20 Polyclonal antibody (30843-1-AP)

    IF Staining of mouse brain using 30843-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SLC6A20 antibody (30843-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SLC6A20 Polyclonal antibody (30843-1-AP)

    IF Staining of mouse brain using 30843-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SLC6A20 antibody (30843-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SLC6A20 Polyclonal antibody

SLC6A20 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 30843-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Synonyms

XTRP3, XT3, Sodium/imino-acid transporter 1, Sodium- and chloride-dependent transporter XTRP3, SIT1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse kidney tissue, HEK-293 cells, rat kidney tissue
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30843-1-AP targets SLC6A20 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC6A20 fusion protein Ag33967 相同性解析による交差性が予測される生物種
Full Name solute carrier family 6 (proline IMINO transporter), member 20
Observed molecular weight 50-60 kDa
GenBank accession numberBC136431
Gene Symbol SLC6A20
Gene ID (NCBI) 54716
RRIDAB_3669764
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9NP91
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC6A20 also known as XT3, SIT1, belongs to the family of solute carrier 6, mediating the transport of neutral amino acids from the extracellular milieu toward cell or storage vesicles utilizing an electric membrane potential as the driving force. SLC6A20 is expressed in the kidney and small intestine and is localized in the brush marginal membrane of the proximal renal tubules (PMID: 16174864). Mutations in SLC6A20 are associated with Hyperglycinuria and Iminoglycinuria (PMID: 19033659)

Protocols

Product Specific Protocols
WB protocol for SLC6A20 antibody 30843-1-APDownload protocol
IF protocol for SLC6A20 antibody 30843-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols