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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Serotonin transporter Polyclonal antibody (29186-1-AP)

    WB analysis using 29186-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29186-1-AP (Serotonin transporter antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Serotonin transporter Polyclonal antibody

Serotonin transporter Polyclonal Antibody for WB, ELISA
Cat No. 29186-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, ELISA

Synonyms

5 HTT, 5 HTTLPR, 5HT transporter, 5HTT, hSERT, HTT, OCD1, serotonin transporter, SERT, SLC6A4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells, RAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29186-1-AP targets Serotonin transporter in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Serotonin transporter fusion protein Ag30412 相同性解析による交差性が予測される生物種
Full Name solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
Calculated molecular weight 70 aa
Observed molecular weight70 kDa
GenBank accession numberNM_001045
Gene Symbol Serotonin transporter
Gene ID (NCBI) 6532
RRIDAB_2935471
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31645
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Serotonin transporter (SERT; SLC6A4) belongs to the sodium- and chloride-dependent monoamine transporter family. SERT is a membrane transporter that terminates the neurotransmission of serotonin, a monoaminergic neurotransmitter, through its reuptake. The SERT uptake mechanism acquires energy for active transport via ATP hydrolysis or via movement against electrochemical gradients. As an oligomeric N-glycan, SERT contains disulfide bonds between cysteine residues on the second extracellular domain. Post-translational modifications regulate the uptake kinetics and membrane trafficking of SERT, in part by regulating the proper folding and assembly of SERT in a host-dependent manner (PMID: 30394319).

Protocols

Product Specific Protocols
WB protocol for Serotonin transporter antibody 29186-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols