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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC7A11/xCT Polyclonal antibody (32384-1-AP)

    WB analysis using 32384-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32384-1-AP (SLC7A11/xCT antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SLC7A11/xCT Polyclonal antibody (32384-1-AP)

    WB analysis using 32384-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32384-1-AP (SLC7A11/xCT antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using SLC7A11/xCT Polyclonal antibody (32384-1-AP)

    IF Staining of mouse eye using 32384-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using SLC7A11/xCT antibody (32384-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using SLC7A11/xCT Polyclonal antibody (32384-1-AP)

    IF Staining of mouse eye using 32384-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using SLC7A11/xCT antibody (32384-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SLC7A11/xCT Polyclonal antibody

SLC7A11/xCT Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 32384-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Synonyms

sut, Subtle gray protein, Solute carrier family 7 member 11, Slc7a11, Cystine/glutamate transporter

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, HEK-293 cells, HeLa cells, HepG2 cells, rat brain tissue, mouse kidney tissue, mouse stomach tissue, rat liver tissue
Positive IF-P detected inmouse eye tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32384-1-AP targets SLC7A11/xCT in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC7A11/xCT fusion protein Ag38215 相同性解析による交差性が予測される生物種
Full Name solute carrier family 7 (cationic amino acid transporter, y+ system), member 11
Calculated molecular weight55 kDa
Observed molecular weight70 kDa, 45 kDa
GenBank accession numberNM_011990
Gene Symbol Slc7a11
Gene ID (NCBI) 26570
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9WTR6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC7A11 (also known as xCT) is a membrane transporter involved in cysteine uptake. It promotes glutathione synthesis through cystine uptake and the concomitant release of glutamate. This, in turn, protects cells from oxidative stress, maintains cellular redox balance, and prevents cell death induced by lipid peroxidation. SLC7A11 has been identified as a potential target for cancer therapeutics. It is overexpressed in multiple malignant tumors and is closely associated with the growth, prognosis, metastasis, and treatment response of various cancers, including lung cancer. Although different from the predicted size of 55 kDa, the 45- and 70-kDa bands represent xCT, most likely a monomer and homodimer of the protein (PMID: 16399997).

Protocols

Product Specific Protocols
WB protocol for SLC7A11/xCT antibody 32384-1-APDownload protocol
IF protocol for SLC7A11/xCT antibody 32384-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols