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SLC7A5 Polyclonal antibody

Name: SLC7A5 Polyclonal antibody
Brand: Proteintech
SKU: 28670-1-AP
Rating: 5.00 (1 reviews)

SLC7A5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 28670-1-AP

Synonyms

D16S469E, CD98LC, CD98 light chain, 4F2LC, 4F2 light chain

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 28670-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 28670-1-AP (SLC7A5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 28670-1-AP
WB result of SLC7A5 antibody (28670-1-AP; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC7A5 transfected MCF-7 cells.
WB analysis of MCF-7 using 28670-1-AP
WB result of SLC7A5 antibody (28670-1-AP; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC7A5 transfected MCF-7 cells.
IHC staining of human lung cancer using 28670-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 28670-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 28670-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 28670-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 28670-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 28670-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HT-29 using 28670-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using SLC7A5 antibody (28670-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 28670-1-AP

Size/Concentration

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A549 cells, MCF-7 cells, HeLa cells, HepG2 cells, MKN-45 cells, SW480 cells
Positive IHC detected in	human lung cancer tissue, human placenta tissue, human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HT-29 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 8 publications below
IHC	See 2 publications below

Product Information

28670-1-AP targets SLC7A5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SLC7A5 fusion protein Ag29164 相同性解析による交差性が予測される生物種
Full Name	solute carrier family 7 (cationic amino acid transporter, y+ system), member 5
Calculated molecular weight	507 aa, 55 kDa
Observed molecular weight	35-40 kDa
GenBank accession number	BC039692
Gene symbol	SLC7A5
Gene ID (NCBI)	8140
RRID	AB_2918188
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Large neutral amino acid transporter 1 LAT1(also named as SLC7A5) is a sodium- and pH-independent transporter that supplies essential amino acids (e.g., leucine, phenylalanine) to cells. It plays an important role in the blood-brain barrier (BBB), where it facilitates the transport of thyroid hormones, drugs (e.g., l-DOPA, gabapentin), and metabolites to the brain. In addition, its expression is highly upregulated in various types of human cancers, which are characterized by a high demand for amino acids for growth and proliferation. The LAT1 subunit in humans is a 507 amino acid-long polypeptide with a theoretical molecular mass of 55 kDa. The protein is hydrophobic and is predicted to be constituted by 12 transmembrane segments. The apparent molecular mass of the proteins diminished to approximately 35 - 40 kDa.(PMID: 23912240 ;PMID: 26256001)

Protocols

Product Specific Protocols
WB protocol for SLC7A5 antibody 28670-1-AP	Download protocol
IHC protocol for SLC7A5 antibody 28670-1-AP	Download protocol
IF protocol for SLC7A5 antibody 28670-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	J Inherit Metab Dis Metabolic characterization of neurogenetic disorders involving glutamatergic neurotransmission Authors - Sofía Illescas View Article
human	WB	J Exp Clin Cancer Res CircARID1A binds to IGF2BP3 in gastric cancer and promotes cancer proliferation by forming a circARID1A-IGF2BP3-SLC7A5 RNA-protein ternary complex Authors - Qiang Ma View Article
human	IHC,WB	Front Oncol ZNF24 regulates the progression of KRAS mutant lung adenocarcinoma by promoting SLC7A5 translation Authors - Daqi Jia View Article
human	WB	Sci Adv Arginine limitation drives a directed codon-dependent DNA sequence evolution response in colorectal cancer cells Authors - Dennis J Hsu View Article
human	WB,IHC	J Exp Clin Cancer Res FBW7/GSK3β mediated degradation of IGF2BP2 inhibits IGF2BP2-SLC7A5 positive feedback loop and radioresistance in lung cancer Authors - Zhiyuan Zhou View Article
mouse	WB	Nat Cell Biol A CRISPRi/a screening platform to study cellular nutrient transport in diverse microenvironments Authors - Christopher Chidley View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Xie (Verified Customer) (07-07-2023)

The expression of Slc7a5 is good for my samples

Applications: Immunofluorescence
Primary Antibody Dilution: 1:250
Cell Tissue Type: brain

SLC7A5 Antibody Immunofluorescence validation (1:250 dilution) in brain (Cat no:28670-1-AP)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC7A5 Polyclonal antibody

SLC7A5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 28670-1-AP

WB analysis of MCF-7 using 28670-1-AP

WB analysis of MCF-7 using 28670-1-AP

IHC staining of human lung cancer using 28670-1-AP

IHC staining of human stomach cancer using 28670-1-AP

IHC staining of human stomach cancer using 28670-1-AP

IHC staining of human placenta using 28670-1-AP

IHC staining of human placenta using 28670-1-AP

IHC staining of human lung cancer using 28670-1-AP

IF Staining of HT-29 using 28670-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Metabolic characterization of neurogenetic disorders involving glutamatergic neurotransmission

CircARID1A binds to IGF2BP3 in gastric cancer and promotes cancer proliferation by forming a circARID1A-IGF2BP3-SLC7A5 RNA-protein ternary complex

ZNF24 regulates the progression of KRAS mutant lung adenocarcinoma by promoting SLC7A5 translation

Arginine limitation drives a directed codon-dependent DNA sequence evolution response in colorectal cancer cells

FBW7/GSK3β mediated degradation of IGF2BP2 inhibits IGF2BP2-SLC7A5 positive feedback loop and radioresistance in lung cancer

A CRISPRi/a screening platform to study cellular nutrient transport in diverse microenvironments

Reviews