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  • KD/KO Validated

SLC7A5 Polyclonal antibody

SLC7A5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28670-1-AP

Synonyms

D16S469E, CD98LC, CD98 light chain, 4F2LC, 4F2 light chain


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Tested Applications

Positive WB detected inA549 cells, MCF-7 cells, HeLa cells, HepG2 cells, MKN-45 cells, SW480 cells
Positive IHC detected inhuman lung cancer tissue, human placenta tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHT-29 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28670-1-AP targets SLC7A5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC7A5 fusion protein Ag29164 相同性解析による交差性が予測される生物種
Full Name solute carrier family 7 (cationic amino acid transporter, y+ system), member 5
Calculated molecular weight 507 aa, 55 kDa
Observed molecular weight35-40 kDa
GenBank accession numberBC039692
Gene symbol SLC7A5
Gene ID (NCBI) 8140
RRIDAB_2918188
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Large neutral amino acid transporter 1 LAT1(also named as SLC7A5) is a sodium- and pH-independent transporter that supplies essential amino acids (e.g., leucine, phenylalanine) to cells. It plays an important role in the blood-brain barrier (BBB), where it facilitates the transport of thyroid hormones, drugs (e.g., l-DOPA, gabapentin), and metabolites to the brain. In addition, its expression is highly upregulated in various types of human cancers, which are characterized by a high demand for amino acids for growth and proliferation. The LAT1 subunit in humans is a 507 amino acid-long polypeptide with a theoretical molecular mass of 55 kDa. The protein is hydrophobic and is predicted to be constituted by 12 transmembrane segments. The apparent molecular mass of the proteins diminished to approximately 35 - 40 kDa.(PMID: 23912240 ;PMID: 26256001)

Protocols

Product Specific Protocols
WB protocol for SLC7A5 antibody 28670-1-APDownload protocol
IHC protocol for SLC7A5 antibody 28670-1-APDownload protocol
IF protocol for SLC7A5 antibody 28670-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Inherit Metab Dis

Metabolic characterization of neurogenetic disorders involving glutamatergic neurotransmission

Authors - Sofía Illescas
humanWB

J Exp Clin Cancer Res

CircARID1A binds to IGF2BP3 in gastric cancer and promotes cancer proliferation by forming a circARID1A-IGF2BP3-SLC7A5 RNA-protein ternary complex

Authors - Qiang Ma
humanIHC,WB

Front Oncol

ZNF24 regulates the progression of KRAS mutant lung adenocarcinoma by promoting SLC7A5 translation

Authors - Daqi Jia
humanWB

Sci Adv

Arginine limitation drives a directed codon-dependent DNA sequence evolution response in colorectal cancer cells

Authors - Dennis J Hsu
humanWB,IHC

J Exp Clin Cancer Res

FBW7/GSK3β mediated degradation of IGF2BP2 inhibits IGF2BP2-SLC7A5 positive feedback loop and radioresistance in lung cancer

Authors - Zhiyuan Zhou
mouseWB

Nat Cell Biol

A CRISPRi/a screening platform to study cellular nutrient transport in diverse microenvironments

Authors - Christopher Chidley

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Xie (Verified Customer) (07-07-2023)

The expression of Slc7a5 is good for my samples

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: brain
SLC7A5 Antibody Immunofluorescence validation (1:250 dilution) in brain (Cat no:28670-1-AP)