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  • Featured Product
  • KD/KO Validated

SLC7A5 Polyclonal antibody

SLC7A5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28670-1-AP

Synonyms

LAT1, D16S469E, CD98LC, CD98 light chain, 4F2LC

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using SLC7A5 Polyclonal antibody (28670-1-AP)

    WB analysis using 28670-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28670-1-AP (SLC7A5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using SLC7A5 Polyclonal antibody (28670-1-AP)

    WB analysis of MCF-7 using 28670-1-AP

    WB result of SLC7A5 antibody (28670-1-AP; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC7A5 transfected MCF-7 cells.

  • Western Blot (WB) analysis of MCF-7 cells using SLC7A5 Polyclonal antibody (28670-1-AP)

    WB analysis of MCF-7 using 28670-1-AP

    WB result of SLC7A5 antibody (28670-1-AP; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC7A5 transfected MCF-7 cells.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SLC7A5 Polyclonal antibody (28670-1-AP)

    IHC staining of human lung cancer using 28670-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using SLC7A5 Polyclonal antibody (28670-1-AP)

    IHC staining of human placenta using 28670-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using SLC7A5 Polyclonal antibody (28670-1-AP)

    IHC staining of human placenta using 28670-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using SLC7A5 Polyclonal antibody (28670-1-AP)

    IHC staining of human placenta using 28670-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using SLC7A5 Polyclonal antibody (28670-1-AP)

    IHC staining of human placenta using 28670-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SLC7A5 Polyclonal antibody (28670-1-AP)

    IHC staining of human stomach cancer using 28670-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SLC7A5 Polyclonal antibody (28670-1-AP)

    IHC staining of human stomach cancer using 28670-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SLC7A5 Polyclonal antibody (28670-1-AP)

    IHC staining of human lung cancer using 28670-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using SLC7A5 Polyclonal antibody (28670-1-AP)

    IF Staining of HT-29 using 28670-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HT-29 cells using SLC7A5 antibody (28670-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using SLC7A5 Polyclonal antibody (28670-1-AP)

    IF Staining of HT-29 using 28670-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using SLC7A5 antibody (28670-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, MCF-7 cells, HeLa cells, HepG2 cells, MKN-45 cells, SW480 cells
Positive IHC detected inhuman lung cancer tissue, human placenta tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHT-29 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28670-1-AP targets SLC7A5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC7A5 fusion protein Ag29164 相同性解析による交差性が予測される生物種
Full Name solute carrier family 7 (cationic amino acid transporter, y+ system), member 5
Calculated molecular weight 507 aa, 55 kDa
Observed molecular weight35-40 kDa
GenBank accession numberBC039692
Gene symbol SLC7A5
Gene ID (NCBI) 8140
RRIDAB_2918188
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Large neutral amino acid transporter 1 LAT1(also named as SLC7A5) is a sodium- and pH-independent transporter that supplies essential amino acids (e.g., leucine, phenylalanine) to cells. It plays an important role in the blood-brain barrier (BBB), where it facilitates the transport of thyroid hormones, drugs (e.g., l-DOPA, gabapentin), and metabolites to the brain. In addition, its expression is highly upregulated in various types of human cancers, which are characterized by a high demand for amino acids for growth and proliferation. The LAT1 subunit in humans is a 507 amino acid-long polypeptide with a theoretical molecular mass of 55 kDa. The protein is hydrophobic and is predicted to be constituted by 12 transmembrane segments. The apparent molecular mass of the proteins diminished to approximately 35 - 40 kDa.(PMID: 23912240 ;PMID: 26256001)

Protocols

Product Specific Protocols
WB protocol for SLC7A5 antibody 28670-1-APDownload protocol
IHC protocol for SLC7A5 antibody 28670-1-APDownload protocol
IF protocol for SLC7A5 antibody 28670-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Eur J Nucl Med Mol Imaging

Synthesis, preclinical assessment, and first-in-human study of [18F]d4-FET for brain tumor imaging

Authors - Lu Hou
View Article
humanWB

Mol Metab

RNA-binding protein YBX3 promotes PPARγ-SLC3A2 mediated BCAA metabolism fueling brown adipogenesis and thermogenesis

Authors - Lin-Yun Chen
View Article
mouseWB

J Inherit Metab Dis

Metabolic characterization of neurogenetic disorders involving glutamatergic neurotransmission

Authors - Sofía Illescas
View Article
humanWB

Oncol Lett

SLC7A5 regulates tryptophan uptake and PD‑L1 expression levels via the kynurenine pathway in ovarian cancer

Authors - Ruibin Jiang
View Article
humanWB

J Exp Clin Cancer Res

CircARID1A binds to IGF2BP3 in gastric cancer and promotes cancer proliferation by forming a circARID1A-IGF2BP3-SLC7A5 RNA-protein ternary complex

Authors - Qiang Ma
View Article
humanIHC,WB

Front Oncol

ZNF24 regulates the progression of KRAS mutant lung adenocarcinoma by promoting SLC7A5 translation

Authors - Daqi Jia
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Xie (Verified Customer) (07-07-2023)

The expression of Slc7a5 is good for my samples

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: brain
SLC7A5 Antibody Immunofluorescence validation (1:250 dilution) in brain (Cat no:28670-1-AP)