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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using SLC7A5 Recombinant antibody (84178-5-RR)

    WB analysis of A549 using 84178-5-RR (same clone as 84178-5-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 84178-5-RR (SLC7A5 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84178-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using SLC7A5 Recombinant antibody (84178-5-RR)

    IF Staining of HT-29 using 84178-5-RR (same clone as 84178-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using SLC7A5 antibody (84178-5-RR, Clone: 241476D5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84178-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HT-29 cells using SLC7A5 Recombinant antibody (84178-5-RR)

    FC experiment of HT-29 using 84178-5-RR (same clone as 84178-5-PBS)

    1x10^6 HT-29 cells were intracellularly stained with 0.25 ug SLC7A5 Recombinant antibody (84178-5-RR, Clone:241476D5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84178-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84178-5-RR

    Affinity and Kinetic Characterization of 84178-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84178-5-RR against Human SLC7A5 were performed. The affinity constant is 0.319 nM.

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SLC7A5 Recombinant antibody, PBS Only

SLC7A5 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84178-5-PBS
Clone No.241476D5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

LAT1, CD98LC, CD98 light chain, 4F2LC, 4F2 light chain

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84178-5-PBS targets SLC7A5 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SLC7A5 fusion protein Ag29164 相同性解析による交差性が予測される生物種
Full Name solute carrier family 7 (cationic amino acid transporter, y+ system), member 5
Calculated molecular weight 507 aa, 55 kDa
Observed molecular weight35-40 kDa
GenBank accession numberBC039692
Gene Symbol SLC7A5
Gene ID (NCBI) 8140
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ01650
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Large neutral amino acid transporter 1 LAT1(also named as SLC7A5) is a sodium- and pH-independent transporter that supplies essential amino acids (e.g., leucine, phenylalanine) to cells. It plays an important role in the blood-brain barrier (BBB), where it facilitates the transport of thyroid hormones, drugs (e.g., l-DOPA, gabapentin), and metabolites to the brain. In addition, its expression is highly upregulated in various types of human cancers, which are characterized by a high demand for amino acids for growth and proliferation. The LAT1 subunit in humans is a 507 amino acid-long polypeptide with a theoretical molecular mass of 55 kDa. The protein is hydrophobic and is predicted to be constituted by 12 transmembrane segments. The apparent molecular mass of the proteins diminished to approximately 35 - 40 kDa.(PMID: 23912240 ;PMID: 26256001)