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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC7A6 Polyclonal antibody

SLC7A6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13823-1-AP

Synonyms

KIAA0245, LAT 2, LAT3, SLC7A6, Y+L amino acid transporter 2, y+LAT 2, Y+LAT2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC7A6 Polyclonal antibody (13823-1-AP)

    WB analysis using 13823-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13823-1-AP (SLC7A6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using SLC7A6 Polyclonal antibody (13823-1-AP)

    WB analysis of NIH/3T3 using 13823-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13823-1-AP (SLC7A6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SLC7A6 Polyclonal antibody (13823-1-AP)

    IHC staining of human colon cancer using 13823-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13823-1-AP (SLC7A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SLC7A6 Polyclonal antibody (13823-1-AP)

    IHC staining of human prostate cancer using 13823-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13823-1-AP (SLC7A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SLC7A6 Polyclonal antibody (13823-1-AP)

    IHC staining of human prostate cancer using 13823-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13823-1-AP (SLC7A6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SLC7A6 Polyclonal antibody (13823-1-AP)

    IHC staining of human colon cancer using 13823-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13823-1-AP (SLC7A6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHUVEC cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

13823-1-AP targets SLC7A6 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitychicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC7A6 fusion protein Ag4924 相同性解析による交差性が予測される生物種
Full Name solute carrier family 7 (cationic amino acid transporter, y+ system), member 6
Calculated molecular weight 515 aa, 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC028216
Gene symbol SLC7A6
Gene ID (NCBI) 9057
RRIDAB_2877978
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SLC7A6 antibody 13823-1-APDownload protocol
IHC protocol for SLC7A6 antibody 13823-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
chickenWB

Front Physiol

Organic zinc with moderate chelation strength enhances zinc absorption in the small intestine and expression of related transporters in the duodenum of broilers

Authors - Yun Hu
View Article
chickenWB

J Anim Sci

Zinc proteinate with moderate chelation strength enhances zinc absorption by upregulating the expression of zinc and amino acid transporters in primary cultured duodenal epithelial cells of broiler embryos

Authors - Yun Hu
View Article
chickenWB

Poult Sci

The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers

Authors - Yun Hu
View Article