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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC7A8 Polyclonal antibody (30211-1-AP)

    WB analysis using 30211-1-AP

    JAR cells were subjected to SDS PAGE followed by western blot with 30211-1-AP (SLC7A8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SLC7A8 Polyclonal antibody (30211-1-AP)

    WB analysis using 30211-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30211-1-AP (SLC7A8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SLC7A8 Polyclonal antibody (30211-1-AP)

    IF Staining of HeLa using 30211-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SLC7A8 antibody (30211-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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SLC7A8 Polyclonal antibody

SLC7A8 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30211-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Solute carrier family 7 member 8, LAT2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman placenta tissue, JAR cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30211-1-AP targets SLC7A8 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC7A8 fusion protein Ag32957 相同性解析による交差性が予測される生物種
Full Name solute carrier family 7 (cationic amino acid transporter, y+ system), member 8
Calculated molecular weight58 kDa
Observed molecular weight45-50 kDa
Gene Symbol SLC7A8
Gene ID (NCBI) 23428
RRIDAB_3086264
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHI5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Solute carrier family 7 member 8 (SLC7A8), also known as LAT2, is an amino acid transporter that, together with LAT1 (SLC7A5), facilitates the bidirectional transport of branched-chain and aromatic AAs across the plasma membrane.

Protocols

Product Specific Protocols
WB protocol for SLC7A8 antibody 30211-1-APDownload protocol
IF protocol for SLC7A8 antibody 30211-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols