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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC9A3R2 Polyclonal antibody

SLC9A3R2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18891-1-AP

Synonyms

NHERF-2, NHERF2, NHERF 2, NHE3RF2, Na(+)/H(+) exchange regulatory cofactor NHE-RF2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC9A3R2 Polyclonal antibody (18891-1-AP)

    WB analysis using 18891-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18891-1-AP (SLC9A3R2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SLC9A3R2 Polyclonal antibody (18891-1-AP)

    IHC staining of human stomach cancer using 18891-1-AP

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 18891-1-AP (SLC9A3R2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, T-47D cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18891-1-AP targets SLC9A3R2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC9A3R2 fusion protein Ag13476 相同性解析による交差性が予測される生物種
Full Name solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2
Calculated molecular weight 337 aa, 37 kDa
Observed molecular weight45 kDa
GenBank accession numberBC106001
Gene symbol SLC9A3R2
Gene ID (NCBI) 9351
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC9A3R2, also named as NHERF2, is a member of the Na+/H+ exchanger regulatory factor (NHERF) family of PDZ scaffolding proteins. SLC9A3R2 is needed for cGMP and carbachol-mediated inhibition as well as LPA and dexamethasone-mediated stimulation of NHE3. NHERF1 and NHERF2 are highly homologous structurally to each other but play different roles in the regulation of Na+ and Cl− transport.(PMID: 23612977)

Protocols

Product Specific Protocols
WB protocol for SLC9A3R2 antibody 18891-1-APDownload protocol
IHC protocol for SLC9A3R2 antibody 18891-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols