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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC9A3R2 Polyclonal antibody (18891-1-AP)

    WB analysis using 18891-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18891-1-AP (SLC9A3R2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SLC9A3R2 Polyclonal antibody (18891-1-AP)

    IHC staining of human stomach cancer using 18891-1-AP

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 18891-1-AP (SLC9A3R2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SLC9A3R2 Polyclonal antibody

SLC9A3R2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18891-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

NHERF-2, NHERF2, NHERF 2, NHE3RF2, Na(+)/H(+) exchange regulatory cofactor NHE-RF2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, T-47D cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18891-1-AP targets SLC9A3R2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC9A3R2 fusion protein Ag13476 相同性解析による交差性が予測される生物種
Full Name solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2
Calculated molecular weight 337 aa, 37 kDa
Observed molecular weight45 kDa
GenBank accession numberBC106001
Gene Symbol SLC9A3R2
Gene ID (NCBI) 9351
RRIDAB_3085570
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15599
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC9A3R2, also named as NHERF2, is a member of the Na+/H+ exchanger regulatory factor (NHERF) family of PDZ scaffolding proteins. SLC9A3R2 is needed for cGMP and carbachol-mediated inhibition as well as LPA and dexamethasone-mediated stimulation of NHE3. NHERF1 and NHERF2 are highly homologous structurally to each other but play different roles in the regulation of Na+ and Cl− transport.(PMID: 23612977)

Protocols

Product Specific Protocols
WB protocol for SLC9A3R2 antibody 18891-1-APDownload protocol
IHC protocol for SLC9A3R2 antibody 18891-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols