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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC9A3R2 Recombinant antibody

SLC9A3R2 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241215A3

Cat no : 84060-5-RR

Synonyms

NHERF2, NHERF 2, NHE3RF2, Na(+)/H(+) exchange regulatory cofactor NHE-RF2, E3KARP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC9A3R2 Recombinant antibody (84060-5-RR)

    WB analysis using 84060-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84060-5-RR (SLC9A3R2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inT-47D cells, MCF-7 cells, HeLa cells, HEK-293T cells, U-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84060-5-RR is SLC9A3R2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SLC9A3R2 fusion protein Ag13476 相同性解析による交差性が予測される生物種
Full Name solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2
Calculated molecular weight 337 aa, 37 kDa
Observed molecular weight~45 kDa
GenBank accession numberBC106001
Gene symbol SLC9A3R2
Gene ID (NCBI) 9351
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC9A3R2, also named as NHERF2, is a member of the Na+/H+ exchanger regulatory factor (NHERF) family of PDZ scaffolding proteins. SLC9A3R2 is needed for cGMP and carbachol-mediated inhibition as well as LPA and dexamethasone-mediated stimulation of NHE3. NHERF1 and NHERF2 are highly homologous structurally to each other but play different roles in the regulation of Na+ and Cl− transport.(PMID: 23612977)

Protocols

Product Specific Protocols
WB protocol for SLC9A3R2 antibody 84060-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols