Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of Raji using 13718-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 13718-1-AP (SLC9A9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 13718-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 13718-1-AP (SLC9A9 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13718-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13718-1-AP (SLC9A9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human tonsillitis using 13718-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13718-1-AP (SLC9A9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 13718-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13718-1-AP (SLC9A9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 13718-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13718-1-AP (SLC9A9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 13718-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13718-1-AP (SLC9A9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 13718-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13718-1-AP (SLC9A9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 13718-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 13718-1-AP (SLC9A9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

SLC9A9 Polyclonal antibody

SLC9A9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13718-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Synonyms

NHE-9, NHE9, NHE 9, Nbla00118, Na(+)/H(+) exchanger 9

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Raji cells, HeLa cells, HepG2 cells
Positive IHC detected in	human tonsillitis tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:600
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
CoIP	See 1 publications below

Product Information

13718-1-AP targets SLC9A9 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SLC9A9 fusion protein Ag4660 相同性解析による交差性が予測される生物種
Full Name	solute carrier family 9 (sodium/hydrogen exchanger), member 9
Calculated molecular weight	645 aa, 73 kDa
Observed molecular weight	66-73 kDa
GenBank accession number	BC035779
Gene Symbol	SLC9A9
Gene ID (NCBI)	285195
RRID	AB_2189452
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8IVB4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for SLC9A9 antibody 13718-1-AP	Download protocol
IHC protocol for SLC9A9 antibody 13718-1-AP	Download protocol
IF protocol for SLC9A9 antibody 13718-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Commun Signal MicroRNA-135a regulates NHE9 to inhibit proliferation and migration of glioblastoma cells. Authors - Daniela M Gomez Zubieta View Article
human	WB	Am J Med Genet B Neuropsychiatr Genet Autism spectrum disorder traits in Slc9a9 knock-out mice. Authors - Lina Yang View Article
human	WB, CoIP	Atten Defic Hyperact Disord Effect of disease-associated SLC9A9 mutations on protein-protein interaction networks: implications for molecular mechanisms for ADHD and autism. Authors - Yanli Zhang-James View Article
human	WB	Mol Cancer Res An Endosomal Acid-Regulatory Feedback System Rewires Cytosolic cAMP Metabolism and Drives Tumor Progression Authors - Hari Prasad View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Raji using 13718-1-AP

WB analysis of HepG2 using 13718-1-AP

WB analysis of HeLa using 13718-1-AP

IHC staining of human tonsillitis using 13718-1-AP

IHC staining of human tonsillitis using 13718-1-AP

IHC staining of human liver cancer using 13718-1-AP

IHC staining of human liver cancer using 13718-1-AP

IHC staining of human tonsillitis using 13718-1-AP

IF Staining of HepG2 using 13718-1-AP

SLC9A9 Polyclonal antibody

SLC9A9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

MicroRNA-135a regulates NHE9 to inhibit proliferation and migration of glioblastoma cells.

Autism spectrum disorder traits in Slc9a9 knock-out mice.

Effect of disease-associated SLC9A9 mutations on protein-protein interaction networks: implications for molecular mechanisms for ADHD and autism.

An Endosomal Acid-Regulatory Feedback System Rewires Cytosolic cAMP Metabolism and Drives Tumor Progression