SLC9A9 Polyclonal antibody

Name: SLC9A9 Polyclonal antibody
Brand: Proteintech
SKU: 13718-1-AP

SLC9A9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13718-1-AP

Synonyms

NHE-9, NHE9, NHE 9, Nbla00118, Na(+)/H(+) exchanger 9

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of Raji using 13718-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 13718-1-AP (SLC9A9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 13718-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 13718-1-AP (SLC9A9 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13718-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13718-1-AP (SLC9A9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human tonsillitis using 13718-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13718-1-AP (SLC9A9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 13718-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13718-1-AP (SLC9A9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 13718-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13718-1-AP (SLC9A9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 13718-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13718-1-AP (SLC9A9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 13718-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13718-1-AP (SLC9A9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 13718-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 13718-1-AP (SLC9A9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 13718-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Raji cells, HeLa cells, HepG2 cells
Positive IHC detected in	human tonsillitis tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:600
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
CoIP	See 1 publications below

Product Information

13718-1-AP targets SLC9A9 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SLC9A9 fusion protein Ag4660 相同性解析による交差性が予測される生物種
Full Name	solute carrier family 9 (sodium/hydrogen exchanger), member 9
Calculated molecular weight	645 aa, 73 kDa
Observed molecular weight	66-73 kDa
GenBank accession number	BC035779
Gene symbol	SLC9A9
Gene ID (NCBI)	285195
RRID	AB_2189452
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for SLC9A9 antibody 13718-1-AP	Download protocol
IHC protocol for SLC9A9 antibody 13718-1-AP	Download protocol
IF protocol for SLC9A9 antibody 13718-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Commun Signal MicroRNA-135a regulates NHE9 to inhibit proliferation and migration of glioblastoma cells. Authors - Daniela M Gomez Zubieta View Article
human	WB	Am J Med Genet B Neuropsychiatr Genet Autism spectrum disorder traits in Slc9a9 knock-out mice. Authors - Lina Yang View Article
human	WB, CoIP	Atten Defic Hyperact Disord Effect of disease-associated SLC9A9 mutations on protein-protein interaction networks: implications for molecular mechanisms for ADHD and autism. Authors - Yanli Zhang-James View Article
human	WB	Mol Cancer Res An Endosomal Acid-Regulatory Feedback System Rewires Cytosolic cAMP Metabolism and Drives Tumor Progression Authors - Hari Prasad View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC9A9 Polyclonal antibody

SLC9A9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of Raji using 13718-1-AP

WB analysis of HepG2 using 13718-1-AP

WB analysis of HeLa using 13718-1-AP

IHC staining of human tonsillitis using 13718-1-AP

IHC staining of human tonsillitis using 13718-1-AP

IHC staining of human liver cancer using 13718-1-AP

IHC staining of human liver cancer using 13718-1-AP

IHC staining of human tonsillitis using 13718-1-AP

IF Staining of HepG2 using 13718-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

MicroRNA-135a regulates NHE9 to inhibit proliferation and migration of glioblastoma cells.

Autism spectrum disorder traits in Slc9a9 knock-out mice.

Effect of disease-associated SLC9A9 mutations on protein-protein interaction networks: implications for molecular mechanisms for ADHD and autism.

An Endosomal Acid-Regulatory Feedback System Rewires Cytosolic cAMP Metabolism and Drives Tumor Progression