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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLCO1B3/OATP1B3 Monoclonal antibody, PBS Only

SLCO1B3/OATP1B3 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D9A4

Cat no : 66381-1-PBS

Synonyms

LST 2, LST 3TM13, LST2, LST3, OATP 8, OATP1B3, OATP8, Organic anion transporter 8, SLC21A8, SLCO1B3

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using SLCO1B3/OATP1B3 Monoclonal antibody (66381-1-Ig)

    WB analysis of HepG2 using 66381-1-Ig (same clone as 66381-1-PBS)

    Western blot analysis of SLCO1B3/OATP1B3 in various cell lines using Proteintech antibody 66381-1-Ig at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66381-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLCO1B3/OATP1B3 Monoclonal antibody (66381-1-Ig)

    IHC staining of human liver cancer using 66381-1-Ig (same clone as 66381-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66381-1-Ig (SLCO1B3/OATP1B3 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66381-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLCO1B3/OATP1B3 Monoclonal antibody (66381-1-Ig)

    IHC staining of human liver cancer using 66381-1-Ig (same clone as 66381-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66381-1-Ig (SLCO1B3 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66381-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SLCO1B3/OATP1B3 Monoclonal antibody (66381-1-Ig)

    IF Staining of HepG2 using 66381-1-Ig (same clone as 66381-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SLCO1B3/OATP1B3 antibody (66381-1-Ig, Clone: 1D9A4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66381-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66381-1-PBS targets SLCO1B3/OATP1B3 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SLCO1B3/OATP1B3 fusion protein Ag19033 相同性解析による交差性が予測される生物種
Full Name solute carrier organic anion transporter family, member 1B3
Calculated molecular weight 702 aa, 77 kDa
Observed molecular weight 68-77 kDa
GenBank accession numberBC105597
Gene symbol SLCO1B3
Gene ID (NCBI) 28234
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SLCO1B3, also known as OATP8 or OATP1B3, is a member of Organic Anion Transporting Polypeptides (OATPs) which are sodium-independent organic anion transporters mediating the uptake of a wide range of structurally diverse endogenous and exogenous compounds including bile acids, hormone conjugates, peptides, toxins, as well as a multitude of therapeutic drugs. SLCO1B3 is specifically expressed in liver under normal condition, while its expression has also been observed in cancer tissues like colon, prostate and pancreas. It corresponds to Oatp1b2 in mice, with the gene symbol Slco1b2. The mouse genome encodes only a single transporter gene in the OATP1B subfamily, because SLCO1B1 and SLCO1B3 arose in primates by gene duplication after divergence from rodents. The molecular mass of SLCO1B3 is highly dependent on the glycosylation modification (deglycosylated form of 65 kDa, fully-glycosylated form of 120 kDa).