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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLCO2B1 Polyclonal antibody

SLCO2B1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 55180-1-AP

Synonyms

SLC21A9, OATPRP2, OATPB, OATP2B1, OATP B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLCO2B1 Polyclonal antibody (55180-1-AP)

    WB analysis using 55180-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55180-1-AP (SLCO2B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SLCO2B1 Polyclonal antibody (55180-1-AP)

    WB analysis using 55180-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55180-1-AP (SLCO2B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using SLCO2B1 Polyclonal antibody (55180-1-AP)

    IHC staining of human liver using 55180-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55180-1-AP (SLCO2B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SLCO2B1 Polyclonal antibody (55180-1-AP)

    IF Staining of HepG2 using 55180-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SLCO2B1 antibody (55180-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inMCF-7 cells, HepG2 cells, BV-2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55180-1-AP targets SLCO2B1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name solute carrier organic anion transporter family, member 2B1
Calculated molecular weight 77 kDa
Observed molecular weight61~74 kDa
GenBank accession numberNM_007256
Gene symbol SLCO2B1
Gene ID (NCBI) 11309
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Solute carrier organic anion (SLCO) gene families encode organic anion transport proteins (OATP), which are membrane transporters widely expressed in the human body that influx numerous compounds and drugs including androgens. SLCO2B1 is a member of an organic anion transporter family and has previously been shown to transport a multitude of drugs and steroid hormone conjugates (PMID: 35714613). High expression of SLCO2B1 is known to associate with the resistance to androgen deprivation therapy in prostate cancer (PMID: 29581838).

Protocols

Product Specific Protocols
WB protocol for SLCO2B1 antibody 55180-1-APDownload protocol
IHC protocol for SLCO2B1 antibody 55180-1-APDownload protocol
IF protocol for SLCO2B1 antibody 55180-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols