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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLCO2B1 Recombinant antibody (84353-1-RR)

    WB analysis using 84353-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84353-1-RR (SLCO2B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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SLCO2B1 Recombinant antibody

SLCO2B1 Recombinant Antibody for WB, ELISA
Cat No. 84353-1-RR
Clone No.241199F9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

OATPRP2, OATPB, OATP2B1, OATP B, KIAA0880

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, mouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84353-1-RR targets SLCO2B1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name solute carrier organic anion transporter family, member 2B1
Calculated molecular weight 77 kDa
Observed molecular weight61~74 kDa
GenBank accession numberNM_007256
Gene Symbol SLCO2B1
Gene ID (NCBI) 11309
RRIDAB_3671891
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO94956
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Solute carrier organic anion (SLCO) gene families encode organic anion transport proteins (OATP), which are membrane transporters widely expressed in the human body that influx numerous compounds and drugs including androgens. SLCO2B1 is a member of an organic anion transporter family and has previously been shown to transport a multitude of drugs and steroid hormone conjugates (PMID: 35714613). High expression of SLCO2B1 is known to associate with the resistance to androgen deprivation therapy in prostate cancer (PMID: 29581838). OATP2B1 was insensitive to endoglycosidase H, an enzyme that cleaves only high mannose glycans, as expected for a mature protein undergoing complex glycosylation.

Protocols

Product Specific Protocols
WB protocol for SLCO2B1 antibody 84353-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols