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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLFN11 Polyclonal antibody (26060-1-AP)

    WB analysis using 26060-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26060-1-AP (SLFN11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using SLFN11 Polyclonal antibody (26060-1-AP)

    IP experiment of HEK-293 using 26060-1-AP

    IP result of anti-SLFN11 (IP:26060-1-AP, 4ug; Detection:26060-1-AP 1:800) with HEK-293 cells lysate 1610 ug.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using SLFN11 Polyclonal antibody (26060-1-AP)

    IHC staining of human ovary cancer using 26060-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 26060-1-AP (SLFN11 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using SLFN11 Polyclonal antibody (26060-1-AP)

    IF Staining of A431 using 26060-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using 26060-1-AP (SLFN11 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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SLFN11 Polyclonal antibody

SLFN11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 26060-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Schlafen family member 11, EC:3.1.-.-

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inDU 145 cells, HEK-293 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

26060-1-AP targets SLFN11 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLFN11 fusion protein Ag22030 相同性解析による交差性が予測される生物種
Full Name schlafen family member 11
Calculated molecular weight 901 aa, 103 kDa
Observed molecular weight 103 kDa
GenBank accession numberBC052586
Gene Symbol SLFN11
Gene ID (NCBI) 91607
RRIDAB_2880356
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z7L1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SLFN11 antibody 26060-1-APDownload protocol
IHC protocol for SLFN11 antibody 26060-1-APDownload protocol
IF protocol for SLFN11 antibody 26060-1-APDownload protocol
IP protocol for SLFN11 antibody 26060-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Biol Macromol

Electrochemical immunosensor for the predictive cancer biomarker SLFN11 using reduced graphene oxide/MIL-101(Cr)-NH2 composite

Authors - Pandiyaraj Kanagavalli
View Article