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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLP76 Monoclonal antibody, PBS Only

SLP76 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4H10B1

Cat no : 66465-1-PBS

Synonyms

LCP2, 4H10B1, SLP 76 tyrosine phosphoprotein, SLP 76, SH2 domain-containing leukocyte protein of 76 kDa

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using SLP76 Monoclonal antibody (66465-1-Ig)

    WB analysis of Jurkat using 66465-1-Ig (same clone as 66465-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66465-1-Ig (SLP76 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66465-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MOLT-4 cells using SLP76 Monoclonal antibody (66465-1-Ig)

    WB analysis of MOLT-4 using 66465-1-Ig (same clone as 66465-1-PBS)

    MOLT-4 cells were subjected to SDS PAGE followed by western blot with 66465-1-Ig (SLP76 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66465-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using SLP76 Monoclonal antibody (66465-1-Ig)

    WB analysis of K-562 using 66465-1-Ig (same clone as 66465-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 66465-1-Ig (SLP76 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66465-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SLP76 Monoclonal antibody (66465-1-Ig)

    IF Staining of HeLa using 66465-1-Ig (same clone as 66465-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66465-1-Ig (SLP76 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66465-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using SLP76 Monoclonal antibody (66465-1-Ig)

    FC experiment of Jurkat using 66465-1-Ig (same clone as 66465-1-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human SLP76 (66465-1-Ig, Clone:4H10B1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66465-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66465-1-PBS targets SLP76 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SLP76 fusion protein Ag3432 相同性解析による交差性が予測される生物種
Full Name lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa)
Calculated molecular weight 533 aa, 60 kDa
Observed molecular weight 76 kDa
GenBank accession numberBC016618
Gene symbol SLP76
Gene ID (NCBI) 3937
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

LCP2, also named as SLP76, is an adapter protein that acts as a substrate of the T cell antigen receptor (TCR)-activated protein tyrosine kinase pathway. It contains an N-terminal leucine Zip motif, a tyrosine-rich domain, a proline-rich domain and a C-terminal SH2 domain. It is constitutively associated with the growth factor receptor-bound protein 2 (Grb2)-related adapter protein 2 (Grap2, a.k.a. GADS) through the interaction of its proline residues with one of the SH3 domains of Grap2. SLP76 associates with growth factor receptor bound protein 2 (Grap2), and is thought to play a role TCR-mediated intracellular signal transduction. SLP76 is also expressed by mast cells, and mast cell activation was markedly impaired in SLP76-deficient mice.