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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLTM Polyclonal antibody

SLTM Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17889-1-AP

Synonyms

SAFB-like transcription modulator, Modulator of estrogen-induced transcription, MET

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using SLTM Polyclonal antibody (17889-1-AP)

    WB analysis of mouse brain using 17889-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17889-1-AP (SLTM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SLTM Polyclonal antibody (17889-1-AP)

    WB analysis of HeLa using 17889-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17889-1-AP (SLTM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SLTM Polyclonal antibody (17889-1-AP)

    WB analysis of HeLa using 17889-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17889-1-AP (SLTM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using SLTM Polyclonal antibody (17889-1-AP)

    IHC staining of human skin cancer using 17889-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 17889-1-AP (SLTM antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SLTM Polyclonal antibody (17889-1-AP)

    IF Staining of HepG2 using 17889-1-AP

    Immunofluorescent analysis of HepG2 cells, using SLTM antibody 17889-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17889-1-AP targets SLTM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLTM fusion protein Ag12060 相同性解析による交差性が予測される生物種
Full Name SAFB-like, transcription modulator
Calculated molecular weight478aa,57 kDa; 1034aa,117 kDa
Observed molecular weight 135-140 kDa
GenBank accession numberBC046119
Gene symbol SLTM
Gene ID (NCBI) 79811
RRIDAB_2192729
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLTM, also named as Modulator of estrogen-induced transcription, is a 1034 amino acid protein, which localizes in the nucleus. When it is overexpressed, SLTM acts as a general inhibitor of transcription that eventually leads to apoptosis. The calculated molecular weight of SLTM is 117 kDa, but modified SLTM is about 135-140 kDa.

Protocols

Product Specific Protocols
WB protocol for SLTM antibody 17889-1-APDownload protocol
IHC protocol for SLTM antibody 17889-1-APDownload protocol
IF protocol for SLTM antibody 17889-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols