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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMAD1 Monoclonal antibody, PBS Only

SMAD1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D10B8

Cat no : 66559-1-PBS

Synonyms

BSP 1, BSP1, hSMAD1, JV4 1, JV41, MAD homolog 1, Mad related protein 1, MADH1, MADR1, Mothers against DPP homolog 1, SMAD 1, SMAD family member 1, SMAD1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMAD1 Monoclonal antibody (66559-1-Ig)

    WB analysis using 66559-1-Ig (same clone as 66559-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66559-1-Ig (SMAD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66559-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SMAD1 Monoclonal antibody (66559-1-Ig)

    WB analysis of HeLa using 66559-1-Ig (same clone as 66559-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66559-1-Ig (SMAD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66559-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using SMAD1 Monoclonal antibody (66559-1-Ig)

    IHC staining of human gliomas using 66559-1-Ig (same clone as 66559-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66559-1-Ig (SMAD1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66559-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using SMAD1 Monoclonal antibody (66559-1-Ig)

    IHC staining of human gliomas using 66559-1-Ig (same clone as 66559-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66559-1-Ig (SMAD1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66559-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66559-1-PBS targets SMAD1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SMAD1 fusion protein Ag0701 相同性解析による交差性が予測される生物種
Full Name SMAD family member 1
Calculated molecular weight 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC001878
Gene symbol SMAD1
Gene ID (NCBI) 4086
RRIDAB_2881920
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Transforming growth factor-β (TGF-β) superfamily is recognized as one of the largest families of secreted multifunctional peptides exerting different biological effects on a large variety of cell types, such as regulation of hormone secretion, stimulation of extracellular matrix formation, the inhibition of proliferation of many cell types, cell survival, bone formation, and chemotaxis for inflammatory cells. One of the most important proteins that modulate TGF-β ligand activity is the SMAD family proteins. SMAD1 is one of the receptor-activated Smads. It's also a signal transducers of BMP signaling and binds to several proteins involved in ubiquitin-proteasome system (UPS). Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase