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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMAD2 Monoclonal antibody (67343-1-Ig)

    WB analysis using 67343-1-Ig (same clone as 67343-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67343-1-Ig (SMAD2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67343-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SMAD2 Monoclonal antibody (67343-1-Ig)

    WB analysis using 67343-1-Ig (same clone as 67343-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67343-1-Ig (SMAD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67343-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SMAD2 Monoclonal antibody (67343-1-Ig)

    IHC staining of human liver cancer using 67343-1-Ig (same clone as 67343-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67343-1-Ig (SMAD2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67343-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SMAD2 Monoclonal antibody (67343-1-Ig)

    IHC staining of human liver cancer using 67343-1-Ig (same clone as 67343-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67343-1-Ig (SMAD2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67343-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SMAD2 Monoclonal antibody (67343-1-Ig)

    IF Staining of HepG2 using 67343-1-Ig (same clone as 67343-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67343-1-Ig (SMAD2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67343-1-PBS in a different storage buffer formulation.

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SMAD2 Monoclonal antibody, PBS Only

SMAD2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67343-1-PBS
Clone No.2H10C7

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

hMAD 2, hSMAD2, JV18, JV18 1, MAD homolog 2, Mad related protein 2, MADH2, MADR2, Mothers against DPP homolog 2, SMAD 2, SMAD family member 2, SMAD2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67343-1-PBS targets SMAD2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SMAD2 fusion protein Ag19542 相同性解析による交差性が予測される生物種
Full Name SMAD family member 2
Calculated molecular weight 467 aa, 52 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC014840
Gene Symbol SMAD2
Gene ID (NCBI) 4087
RRIDAB_2882601
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15796
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SMAD2, also named as MADH2 and MADR2, belongs to the dwarfin/SMAD family, contains 1 MH1 (MAD homology 1) domain and 1 MH2 (MAD homology 2) domain. SMAD2 is a receptor-regulated SMAD(R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. This protein may act as a tumor suppressor in colorectal carcinoma. It is phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, It is phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases, and then able to interact with SMURF2, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, it is phosphorylated on Ser-240 by CaMK2. It is phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. In response to TGF-beta, it is ubiquitinated by NEDD4L, which promotes its degradation. In response to TGF-beta signaling, it is acetylated on Lys-19 by coactivators, which increases transcriptional activity. The molecular weight of unphosphorylated forms of Smad2 is 52 kDa and phosphorylated forms of Smad2 is 58 kDa. (PMID: 9006934)