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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMAD2 Recombinant antibody, PBS Only

SMAD2 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240950A11

Cat no : 83841-5-PBS

Synonyms

JV18 1, JV18, hSMAD2, hMAD-2, hMAD 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMAD2 Recombinant antibody (83841-5-RR)

    WB analysis using 83841-5-RR (same clone as 83841-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83841-5-RR (SMAD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83841-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SMAD2 Recombinant antibody (83841-5-RR)

    IF Staining of HepG2 using 83841-5-RR (same clone as 83841-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMAD2 antibody (83841-5-RR, Clone: 240950A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83841-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using SMAD2 Recombinant antibody (83841-5-RR)

    FC experiment of HeLa using 83841-5-RR (same clone as 83841-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SMAD2 Recombinant antibody (83841-5-RR, Clone:240950A11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83841-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using SMAD2 Recombinant antibody (83841-5-RR)

    FC experiment of Jurkat using 83841-5-RR (same clone as 83841-5-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug SMAD2 Recombinant antibody (83841-5-RR, Clone:240950A11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83841-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83841-5-RR

    Affinity and Kinetic Characterization of 83841-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83841-5-RR against Human SMAD2 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83841-5-PBS is SMAD2 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SMAD2 fusion protein Ag3237 相同性解析による交差性が予測される生物種
Full Name SMAD family member 2
Calculated molecular weight 467 aa, 52 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC014840
Gene symbol SMAD2
Gene ID (NCBI) 4087
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SMAD2, also named as MADH2 and MADR2, belongs to the dwarfin/SMAD family, contains 1 MH1 (MAD homology 1) domain and 1 MH2 (MAD homology 2) domain. SMAD2 is a receptor-regulated SMAD(R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. This protein may act as a tumor suppressor in colorectal carcinoma. It is phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, It is phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases, and then able to interact with SMURF2, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, it is phosphorylated on Ser-240 by CaMK2. It is phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. In response to TGF-beta, it is ubiquitinated by NEDD4L, which promotes its degradation. In response to TGF-beta signaling, it is acetylated on Lys-19 by coactivators, which increases transcriptional activity. The molecular weight of unphosphorylated forms of Smad2 is 52 kDa and phosphorylated forms of Smad2 is 58 kDa. (PMID: 9006934)