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Host
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMAD3 Monoclonal antibody, PBS Only

SMAD3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F10B2

Cat no : 66516-1-PBS

Synonyms

hMAD 3, hSMAD3, HSPC193, HsT17436, JV15 2, MAD homolog 3, Mad3, MADH3, Mothers against DPP homolog 3, SMAD 3, SMAD family member 3, SMAD3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using SMAD3 Monoclonal antibody (66516-1-Ig)

    WB analysis using 66516-1-Ig (same clone as 66516-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66516-1-Ig (SMAD3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66516-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SMAD3 Monoclonal antibody (66516-1-Ig)

    WB analysis using 66516-1-Ig (same clone as 66516-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66516-1-Ig (SMAD3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66516-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66516-1-PBS targets SMAD3 in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name SMAD family member 3
Calculated molecular weight 48 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberNM_005902
Gene symbol SMAD3
Gene ID (NCBI) 4088
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

SMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. SMAD3 localizes in the nucleus and cytoplasm. SMAD3 plays an essential role in development and maintenance of self-tolerance and is a critical mediator of the TGFB signaling pathway. SMAD3 is involved in TGFB dependent regulation of steroidogenesis and in T-cell response to TGFB.