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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMAD3 Monoclonal antibody (66516-1-Ig)

    WB analysis using 66516-1-Ig (same clone as 66516-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66516-1-Ig (SMAD3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66516-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SMAD3 Monoclonal antibody (66516-1-Ig)

    WB analysis using 66516-1-Ig (same clone as 66516-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66516-1-Ig (SMAD3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66516-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SMAD3 Monoclonal antibody (66516-1-Ig)

    IHC staining of human stomach cancer using 66516-1-Ig (same clone as 66516-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66516-1-Ig (SMAD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66516-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using SMAD3 Monoclonal antibody (66516-1-Ig)

    IHC staining of mouse lung using 66516-1-Ig (same clone as 66516-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66516-1-Ig (SMAD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66516-1-PBS in a different storage buffer formulation.

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SMAD3 Monoclonal antibody, PBS Only

SMAD3 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66516-1-PBS
Clone No.1F10B2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

JV15 2, HsT17436, HSPC193, hSMAD3, hMAD-3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66516-1-PBS targets SMAD3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name SMAD family member 3
Calculated molecular weight 48 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberNM_005902
Gene Symbol SMAD3
Gene ID (NCBI) 4088
RRIDAB_2881879
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP84022
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. SMAD3 localizes in the nucleus and cytoplasm. SMAD3 plays an essential role in development and maintenance of self-tolerance and is a critical mediator of the TGFB signaling pathway. SMAD3 is involved in TGFB dependent regulation of steroidogenesis and in T-cell response to TGFB.