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KD/KO Validated

SMAD4 Polyclonal antibody

Name: SMAD4 Polyclonal antibody
Brand: Proteintech
SKU: 10231-1-AP

SMAD4 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10231-1-AP

Synonyms

Mothers against DPP homolog 4, MADH4, MAD homolog 4, JIP, hSMAD4

Validation Data Gallery

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AllWBIPIHC

WB analysis using 10231-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10231-1-AP (SMAD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10231-1-AP
WB result of SMAD4 antibody (10231-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMAD4 transfected HEK-293 cells.
WB analysis of NIH/3T3 using 10231-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10231-1-AP (SMAD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 10231-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 10231-1-AP (SMAD4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10231-1-AP
IP result of anti-SMAD4 (IP:10231-1-AP, 4ug; Detection:10231-1-AP 1:1500) with HeLa cells lysate 1320 ug.
IHC staining of human cervical cancer using 10231-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10231-1-AP (SMAD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat small intestine using 10231-1-AP
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 10231-1-AP (SMAD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 10231-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10231-1-AP (SMAD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse pancreas using 10231-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10231-1-AP (SMAD4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse pancreas using 10231-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10231-1-AP (SMAD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat small intestine using 10231-1-AP
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 10231-1-AP (SMAD4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 10231-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10231-1-AP (SMAD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 10231-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10231-1-AP (SMAD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 10231-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	COLO 320 cells, HEK-293 cells, NIH/3T3 cells, mouse liver tissue, HCT 116 cells, HEK-293T cells, Neuro-2a cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human skin cancer tissue, human tonsillitis tissue, human cervical cancer tissue, mouse pancreas tissue, rat small intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 78 publications below
IHC	See 10 publications below
IF	See 7 publications below
IP	See 4 publications below
CoIP	See 1 publications below
ChIP	See 4 publications below

Product Information

10231-1-AP targets SMAD4 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, bovine, hamster, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SMAD4 fusion protein Ag0299 相同性解析による交差性が予測される生物種
Full Name	SMAD family member 4
Calculated molecular weight	60 kDa
Observed molecular weight	63-70 kDa
GenBank accession number	BC002379
Gene symbol	SMAD4
Gene ID (NCBI)	4089
RRID	AB_2193323
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Mammalian homologs of the Drosophila Mad gene include Smad1, Smad2, Smad3, Smad4 (DPC4), Smad5, Smad6, Smad7 and Smad8. Smad1 and Smad5 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interaction with various Smad family members . Smad6 and Smad7 regulate the response to activin/ TGF signaling by interfering with TGF -mediated phosphorylation of other Smad family members. This antibody is a rabbit polyclonal antibody raised against an internal region of human SMAD4.

Protocols

Product Specific Protocols
WB protocol for SMAD4 antibody 10231-1-AP	Download protocol
IHC protocol for SMAD4 antibody 10231-1-AP	Download protocol
IP protocol for SMAD4 antibody 10231-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics Authors - Mai Abdel Mouti View Article
human	WB,IP,IF	Transl Res Deubiquitinase USP13 promotes extracellular matrix expression by stabilizing Smad4 in lung fibroblast cells. Authors - Xinxin Liao KD Validated View Article
human	WB	Theranostics PMID: 28839463 Authors - Junlong Zhuang View Article
rat	WB,IF	Acta Pharmacol Sin Role of thrombospondin-1 in high-salt-induced mesenteric artery endothelial impairment in rats Authors - Fang-Fang Xu KD Validated View Article
human	WB	EMBO Rep Mouse endothelial OTUD1 promotes angiotensin II-induced vascular remodeling by deubiquitinating SMAD3 Authors - Zhuqi Huang View Article
human	WB	Stem Cell Reports Gene Correction Reverses Ciliopathy and Photoreceptor Loss in iPSC-Derived Retinal Organoids from Retinitis Pigmentosa Patients. Authors - Wen-Li Deng View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMAD4 Polyclonal antibody

SMAD4 Polyclonal Antibody for WB, IHC, IP, ELISA

Validation Data Gallery

WB analysis using 10231-1-AP

WB analysis of HEK-293 using 10231-1-AP

WB analysis of NIH/3T3 using 10231-1-AP

WB analysis of mouse liver using 10231-1-AP

IP experiment of HeLa using 10231-1-AP

IHC staining of human cervical cancer using 10231-1-AP

IHC staining of rat small intestine using 10231-1-AP

IHC staining of human skin cancer using 10231-1-AP

IHC staining of mouse pancreas using 10231-1-AP

IHC staining of mouse pancreas using 10231-1-AP

IHC staining of rat small intestine using 10231-1-AP

IHC staining of human tonsillitis using 10231-1-AP

IHC staining of human tonsillitis using 10231-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics

Deubiquitinase USP13 promotes extracellular matrix expression by stabilizing Smad4 in lung fibroblast cells.

PMID: 28839463

Role of thrombospondin-1 in high-salt-induced mesenteric artery endothelial impairment in rats

Mouse endothelial OTUD1 promotes angiotensin II-induced vascular remodeling by deubiquitinating SMAD3

Gene Correction Reverses Ciliopathy and Photoreceptor Loss in iPSC-Derived Retinal Organoids from Retinitis Pigmentosa Patients.