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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMAD4 Recombinant antibody, PBS Only (Detector)

SMAD4 Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240026A4

Cat no : 83169-6-PBS

Synonyms

MADH4, MAD homolog 4, JIP, hSMAD4, DPC4

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00267-4

    Sandwich ELISA standard curve of MP00267-4

    Sandwich ELISA standard curve of MP00267-4, Human SMAD4 Monoclonal Matched Antibody Pair - PBS only. 83169-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0299. 83169-6-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Sample test of MP00267-4

    Sample test of MP00267-4

    The mean human SMAD4 concentration was determined to be 9,362.0 pg/mL in HepG2 cell extract based on a 6.0 mg/mL extract load and 4,272.2 pg/mL in HL-60 cell extract based on a 3 mg/mL extract load.

  • Sample test of MP00267-4

    Sample test of MP00267-4

    Serum of sixteen individual healthy human donors were measured. The human SMAD4 concentration of detected samples was determined to be 47.3 pg/mL with a range of ND - 148.8 pg/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83169-6-PBS targets SMAD4 as part of a matched antibody pair:

MP00267-4: 83169-5-PBS capture and 83169-6-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SMAD4 fusion protein Ag0299 相同性解析による交差性が予測される生物種
Full Name SMAD family member 4
Calculated molecular weight 60 kDa
GenBank accession numberBC002379
Gene symbol SMAD4
Gene ID (NCBI) 4089
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.