• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMAD5 Monoclonal antibody, PBS Only

SMAD5 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A6A4

Cat no : 67052-1-PBS

Synonyms

DKFZp781C1895, DKFZp781O1323, Dwfc, hSmad5, JV5 1, MAD homolog 5, MADH5, Mothers against DPP homolog 5, SMAD 5, SMAD family member 5, SMAD5

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using SMAD5 Monoclonal antibody (67052-1-Ig)

    WB analysis using 67052-1-Ig (same clone as 67052-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67052-1-Ig (SMAD5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67052-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SMAD5 Monoclonal antibody (67052-1-Ig)

    IHC staining of human breast cancer using 67052-1-Ig (same clone as 67052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67052-1-Ig (SMAD5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67052-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SMAD5 Monoclonal antibody (67052-1-Ig)

    IHC staining of human colon cancer using 67052-1-Ig (same clone as 67052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67052-1-Ig (SMAD5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67052-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SMAD5 Monoclonal antibody (67052-1-Ig)

    IHC staining of human colon cancer using 67052-1-Ig (same clone as 67052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67052-1-Ig (SMAD5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67052-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SMAD5 Monoclonal antibody (67052-1-Ig)

    IHC staining of human breast cancer using 67052-1-Ig (same clone as 67052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67052-1-Ig (SMAD5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67052-1-PBS in a different storage buffer formulation.

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67052-1-PBS targets SMAD5 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SMAD5 fusion protein Ag28699 相同性解析による交差性が予測される生物種
Full Name SMAD family member 5
Calculated molecular weight 465 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC009682
Gene symbol SMAD5
Gene ID (NCBI) 4090
RRIDAB_2882365
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SMAD5, also named as MADH5 and JV5-1, is a transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). It plays a critical role in the signaling pathway by which TGF-beta inhibits the proliferation of human hematopoietic progenitor cells.