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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMAD5 Monoclonal antibody (67052-1-Ig)

    WB analysis using 67052-1-Ig (same clone as 67052-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67052-1-Ig (SMAD5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67052-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SMAD5 Monoclonal antibody (67052-1-Ig)

    IHC staining of human breast cancer using 67052-1-Ig (same clone as 67052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67052-1-Ig (SMAD5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67052-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SMAD5 Monoclonal antibody (67052-1-Ig)

    IHC staining of human colon cancer using 67052-1-Ig (same clone as 67052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67052-1-Ig (SMAD5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67052-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SMAD5 Monoclonal antibody (67052-1-Ig)

    IHC staining of human colon cancer using 67052-1-Ig (same clone as 67052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67052-1-Ig (SMAD5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67052-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SMAD5 Monoclonal antibody (67052-1-Ig)

    IHC staining of human breast cancer using 67052-1-Ig (same clone as 67052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67052-1-Ig (SMAD5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67052-1-PBS in a different storage buffer formulation.

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SMAD5 Monoclonal antibody, PBS Only

SMAD5 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67052-1-PBS
Clone No.1A6A4

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

DKFZp781C1895, DKFZp781O1323, Dwfc, hSmad5, JV5 1, MAD homolog 5, MADH5, Mothers against DPP homolog 5, SMAD 5, SMAD family member 5, SMAD5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67052-1-PBS targets SMAD5 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SMAD5 fusion protein Ag28699 相同性解析による交差性が予測される生物種
Full Name SMAD family member 5
Calculated molecular weight 465 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC009682
Gene Symbol SMAD5
Gene ID (NCBI) 4090
RRIDAB_2882365
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99717
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SMAD5, also named as MADH5 and JV5-1, is a transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). It plays a critical role in the signaling pathway by which TGF-beta inhibits the proliferation of human hematopoietic progenitor cells.