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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMAD7 Monoclonal antibody

SMAD7 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3D1C3

Cat no : 68594-1-Ig

Synonyms

MADH7, MAD homolog 8, MAD homolog 7, hSMAD7, CRCS3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMAD7 Monoclonal antibody (68594-1-Ig)

    WB analysis using 68594-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68594-1-Ig (SMAD7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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Tested Applications

Positive WB detected inpig kidney tissue, mouse brain tissue, rat brain tissue, rabbit brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68594-1-Ig targets SMAD7 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SMAD7 fusion protein Ag13688 相同性解析による交差性が予測される生物種
Full Name SMAD family member 7
Calculated molecular weight 426 aa, 46 kDa
Observed molecular weight45-55 kDa
GenBank accession numberBC074819
Gene symbol SMAD7
Gene ID (NCBI) 4092
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMAD7, also named as Mothers against decapentaplegic homolog 7, is a 426 amino acid protein, which belongs to the dwarfin/SMAD family. SMAD7 Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236). SMAD7 as antagonist of signaling by TGF-beta type 1 receptor superfamily members has been shown to inhibit TGF-beta and activin signaling by associating with their receptors thus preventing SMAD2 access. SMAD7 functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex and also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. SMAD7 positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Protocols

Product Specific Protocols
WB protocol for SMAD7 antibody 68594-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols