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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMAP1 Polyclonal antibody

SMAP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12799-1-AP

Synonyms

Stromal membrane-associated protein 1, SMAP 1

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using SMAP1 Polyclonal antibody (12799-1-AP)

    WB analysis of NIH/3T3 using 12799-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12799-1-AP (SMAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human adrenal gland tissue using SMAP1 Polyclonal antibody (12799-1-AP)

    WB analysis of human adrenal gland using 12799-1-AP

    human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 12799-1-AP (SMAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat lymph tissue using SMAP1 Polyclonal antibody (12799-1-AP)

    WB analysis of rat lymph using 12799-1-AP

    rat lymph tissue were subjected to SDS PAGE followed by western blot with 12799-1-AP (SMAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human plasma using SMAP1 Polyclonal antibody (12799-1-AP)

    WB analysis of human plasma using 12799-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 12799-1-AP (SMAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SMAP1 Polyclonal antibody (12799-1-AP)

    WB analysis of mouse brain using 12799-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12799-1-AP (SMAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using SMAP1 Polyclonal antibody (12799-1-AP)

    IHC staining of human stomach using 12799-1-AP

    Immunohistochemical analysis of paraffin-embedded normal stomach slide using 12799-1-AP (SMAP1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using SMAP1 Polyclonal antibody (12799-1-AP)

    IHC staining of human stomach using 12799-1-AP

    Immunohistochemical analysis of paraffin-embedded normal stomach slide using 12799-1-AP (SMAP1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inNIH/3T3 cells, human adrenal gland tissue, human plasma, mouse brain tissue, rat lymph tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12799-1-AP targets SMAP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMAP1 fusion protein Ag3544 相同性解析による交差性が予測される生物種
Full Name small ArfGAP 1
Calculated molecular weight 467 aa, 50 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC036123
Gene symbol SMAP1
Gene ID (NCBI) 60682
RRIDAB_2272651
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMAP1, also named as FLJ13159 and SMAP 1, contains a Arf-GAP domain. SMAP1 is a GTPase activating protein for ARF6 that binds clathrin and regulates clathrin-dependent endocytosis. SMAP1 may play a role in erythropoiesis. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SMAP1.

Protocols

Product Specific Protocols
WB protocol for SMAP1 antibody 12799-1-APDownload protocol
IHC protocol for SMAP1 antibody 12799-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols