Featured Product
KD/KO Validated

SMARCA2-Specific Polyclonal antibody

Name: SMARCA2-Specific Polyclonal antibody
Brand: Proteintech
SKU: 26613-1-AP

SMARCA2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 26613-1-AP

Synonyms

SMARCA2, EC:3.6.4.-, BRM, BRG1-associated factor 190B, BAF190B

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of HeLa using 26613-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 26613-1-AP (SMARCA2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 26613-1-AP
WB result of SMARCA2-Specific antibody (26613-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMARCA2-Specific transfected HeLa cells.
WB analysis of NIH/3T3 using 26613-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26613-1-AP (SMARCA2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of MDA-MB-453s using 26613-1-AP
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 26613-1-AP (SMARCA2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human ovary tumor using 26613-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26613-1-AP (SMARCA2-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 26613-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26613-1-AP (SMARCA2-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 26613-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 26613-1-AP (SMARCA2-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 26613-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMARCA2-Specific antibody (26613-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

-/ -

Cat No. 26613-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, NIH/3T3 cells, MDA-MB-453s cells
Positive IHC detected in	human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
CoIP	See 1 publications below

Product Information

26613-1-AP targets SMARCA2-Specific in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SMARCA2-Specific fusion protein Ag25157 相同性解析による交差性が予測される生物種
Full Name	SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
Calculated molecular weight	181 kDa
Observed molecular weight	180-190 kDa
GenBank accession number	NM_003070
Gene symbol	SMARCA2
Gene ID (NCBI)	6595
RRID	AB_2880575
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SMARCA2 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. SMARCA2 is involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

Protocols

Product Specific Protocols
WB protocol for SMARCA2-Specific antibody 26613-1-AP	Download protocol
IHC protocol for SMARCA2-Specific antibody 26613-1-AP	Download protocol
IF protocol for SMARCA2-Specific antibody 26613-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	Neuron Nucleoporin Seh1 Interacts with Olig2/Brd7 to Promote Oligodendrocyte Differentiation and Myelination. Authors - Zhixiong Liu View Article
human	WB	Oncol Lett PMID: 30127991 Authors - Xiaoli Xu View Article
mouse	WB	Stem Cells Chromatin Remodeling Factor BRG1 Regulates Stemness and Chemosensitivity of Glioma Initiating Cells. Authors - Debolina Ganguly View Article
mouse	WB	Biochem Biophys Res Commun Chromatin remodeler BRM is a key mediator of leucine-stimulated mTOR gene transcription in mouse mammary epithelial cells Authors - Changping Ke View Article
human	CoIP	Nat Commun Prion-like domain mediated phase separation of ARID1A promotes oncogenic potential of Ewing's sarcoma Authors - Yong Ryoul Kim View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMARCA2-Specific Polyclonal antibody

SMARCA2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of HeLa using 26613-1-AP

WB analysis of HeLa using 26613-1-AP

WB analysis of NIH/3T3 using 26613-1-AP

WB analysis of MDA-MB-453s using 26613-1-AP

IHC staining of human ovary tumor using 26613-1-AP

IHC staining of human ovary tumor using 26613-1-AP

IF Staining of HepG2 using 26613-1-AP

IF Staining of HeLa using 26613-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Nucleoporin Seh1 Interacts with Olig2/Brd7 to Promote Oligodendrocyte Differentiation and Myelination.

PMID: 30127991

Chromatin Remodeling Factor BRG1 Regulates Stemness and Chemosensitivity of Glioma Initiating Cells.

Chromatin remodeler BRM is a key mediator of leucine-stimulated mTOR gene transcription in mouse mammary epithelial cells

Prion-like domain mediated phase separation of ARID1A promotes oncogenic potential of Ewing's sarcoma