SMARCA4 Recombinant antibody, PBS Only (Detector)

Various lysates were subjected to SDS PAGE followed by western blot with 83310-2-RR (SMARCA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83310-2-PBS in a different storage buffer formulation.

Sandwich ELISA standard curve of MP00334-1, Human SMARCA4 Recombinant Matched Antibody Pair - PBS only. 83310-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag16256. 83310-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

The mean SMARCA4 concentration was determined to be 22.1 ng/mL in Hela cell extract based on a 3.6 mg/mL extract load and 6.9 ng/mL in HEK-293 cell extract based on a 4.6 mg/mL extract load.

Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with . This data was developed using the same antibody clone with 83310-2-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with . This data was developed using the same antibody clone with 83310-2-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83310-2-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83310-2-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83310-2-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83310-2-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83310-2-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83310-2-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83310-2-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83310-2-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83310-2-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83310-2-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83310-2-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83310-2-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMARCA4 antibody (83310-2-RR, Clone: 240172F4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83310-2-PBS in a different storage buffer formulation.

1x10^6 Jurkat cells were intracellularly stained with 0.25 ug SMARCA4 Recombinant antibody (83310-2-RR, Clone:240172F4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83310-2-PBS in a different storage buffer formulation.

Cytometric bead array standard curve of MP00334-1, SMARCA4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83310-1-PBS. Detection antibody: 83310-2-PBS. Standard: Ag16256. Range: 0.313-40 ng/mL.

Biolayer interferometry (BLl) kinetic assays of 83310-2-RR against Human SMARCA4 were performed. The affinity constant is 3.66 nM.