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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMARCA4 Recombinant antibody (83310-7-RR)

    WB analysis using 83310-7-RR (same clone as 83310-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83310-7-RR (SMARCA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83310-7-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SMARCA4 Recombinant antibody (83310-7-RR)

    IHC staining of human colon cancer using 83310-7-RR (same clone as 83310-7-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83310-7-RR (SMARCA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83310-7-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SMARCA4 Recombinant antibody (83310-7-RR)

    IHC staining of human colon cancer using 83310-7-RR (same clone as 83310-7-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83310-7-RR (SMARCA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83310-7-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SMARCA4 Recombinant antibody (83310-7-RR)

    IHC staining of mouse kidney using 83310-7-RR (same clone as 83310-7-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83310-7-RR (SMARCA4 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83310-7-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using SMARCA4 Recombinant antibody (83310-7-RR)

    FC experiment of Jurkat using 83310-7-RR (same clone as 83310-7-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug SMARCA4 Recombinant antibody (83310-7-RR, Clone:240172F7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83310-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83310-7-RR

    Affinity and Kinetic Characterization of 83310-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83310-7-RR against Human SMARCA4 were performed. The affinity constant is 1.73 nM.

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SMARCA4 Recombinant antibody, PBS Only

SMARCA4 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83310-7-PBS
Clone No.240172F7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

BRG1, BAF190A, BAF190, ATP-dependent helicase SMARCA4, ATP dependent helicase SMARCA4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83310-7-PBS targets SMARCA4 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SMARCA4 fusion protein Ag16256 相同性解析による交差性が予測される生物種
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Calculated molecular weight 1647 aa, 185 kDa
Observed molecular weight 185 kDa
GenBank accession numberBC150298
Gene Symbol SMARCA4
Gene ID (NCBI) 6597
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP51532
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.