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  • Featured Product
  • KD/KO Validated

SMARCA4/BRG1 Polyclonal antibody, PBS Only

SMARCA4/BRG1 Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20650-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SMARCA4/BRG1 Polyclonal antibody (20650-1-AP)

    WB analysis of HeLa using 20650-1-AP (same clone as 20650-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20650-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20650-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using SMARCA4/BRG1 Polyclonal antibody (20650-1-AP)

    WB analysis of HepG2 using 20650-1-AP (same clone as 20650-1-PBS)

    WB result of SMARCA4/BRG1 antibody (20650-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMARCA4/BRG1 transfected HepG2 cells. This data was developed using the same antibody clone with 20650-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using SMARCA4/BRG1 Polyclonal antibody (20650-1-AP)

    WB analysis of K-562 using 20650-1-AP (same clone as 20650-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 20650-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20650-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20650-1-PBS targets SMARCA4/BRG1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Calculated molecular weight 185 kDa
Observed molecular weight 180 kDa
GenBank accession numberNM_001128844
Gene symbol SMARCA4
Gene ID (NCBI) 6597
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. The antibody is specific to SMARCA4.