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CoraLite® Plus 488-conjugated SMARCA4/BRG1 Recombinant antibody

SMARCA4/BRG1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-83310-2
Clone No.240172F4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

SMARCA4, BRG1, BAF190A, BAF190, ATP-dependent helicase SMARCA4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration:  100 μL, 1000 μg/ml
100 μL, 1000 μg/ml

¥85,000/ 100 μL

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テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83310-2 targets SMARCA4/BRG1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SMARCA4/BRG1 fusion protein Ag16256 相同性解析による交差性が予測される生物種
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Calculated molecular weight 1647 aa, 185 kDa
Observed molecular weight 185 kDa
GenBank accession numberBC150298
Gene Symbol SMARCA4
Gene ID (NCBI) 6597
RRIDAB_3673236
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP51532
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SMARCA4/BRG1 antibody CL488-83310-2Download protocol
FC protocol for CL Plus 488 SMARCA4/BRG1 antibody CL488-83310-2Download protocol
Standard Protocols
Click here to view our Standard Protocols
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