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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using SMARCB1 Polyclonal antibody (20654-1-AP)

    WB analysis of HepG2 using 20654-1-AP (same clone as 20654-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20654-1-AP (SMARCB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20654-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using SMARCB1 Polyclonal antibody (20654-1-AP)

    WB analysis of K-562 using 20654-1-AP (same clone as 20654-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 20654-1-AP (SMARCB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20654-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using SMARCB1 Polyclonal antibody (20654-1-AP)

    IP experiment of K-562 using 20654-1-AP (same clone as 20654-1-PBS)

    IP result of anti-SMARCB1 (IP:20654-1-AP, 4ug; Detection:20654-1-AP 1:500) with K-562 cells lysate 3200ug. This data was developed using the same antibody clone with 20654-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using SMARCB1 Polyclonal antibody (20654-1-AP)

    IHC staining of human lymphoma using 20654-1-AP (same clone as 20654-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 20654-1-AP (SMARCB1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20654-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SMARCB1 Polyclonal antibody (20654-1-AP)

    IHC staining of human prostate cancer using 20654-1-AP (same clone as 20654-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20654-1-AP (SMARCB1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20654-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using SMARCB1 Polyclonal antibody (20654-1-AP)

    IHC staining of human lymphoma using 20654-1-AP (same clone as 20654-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 20654-1-AP (SMARCB1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20654-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SMARCB1 Polyclonal antibody (20654-1-AP)

    IHC staining of human prostate cancer using 20654-1-AP (same clone as 20654-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20654-1-AP (SMARCB1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20654-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

SMARCB1 Polyclonal antibody, PBS Only

SMARCB1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 20654-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20654-1-PBS targets SMARCB1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1
Calculated molecular weight 44 kDa
Observed molecular weight40-45 kDa
GenBank accession numberNM_003073
Gene Symbol SMARCB1
Gene ID (NCBI) 6598
RRIDAB_10695761
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12824
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SMARCB1, also named as BAF47, INI1 and SNF5L1, belongs to the SNF5 family. It is a core component of the BAF (hSWI/SNF) complex. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. SMARCB1 stimulates in vitro the remodeling activity of SMARCA4/BRG1/BAF190A. It is involved in activation of CSF1 promoter. SMARCB1 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. SMARCB1 plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Defects in SMARCB1 are a cause of rhabdoid tumor (RDT) which also known as malignant rhabdoid tumor (MRT). Defects in SMARCB1 are a cause of schwannomatosis. The antibody is specific to SMARCB1.