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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMARCC1 Polyclonal antibody

SMARCC1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 17722-1-AP

Synonyms

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1, SRG3, Rsc8, CRACC1, BRG1-associated factor 155

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMARCC1 Polyclonal antibody (17722-1-AP)

    WB analysis using 17722-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17722-1-AP (SMARCC1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat testis tissue using SMARCC1 Polyclonal antibody (17722-1-AP)

    WB analysis of rat testis using 17722-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 17722-1-AP (SMARCC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using SMARCC1 Polyclonal antibody (17722-1-AP)

    WB analysis of Jurkat using 17722-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17722-1-AP (SMARCC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using SMARCC1 Polyclonal antibody (17722-1-AP)

    WB analysis of K-562 using 17722-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 17722-1-AP (SMARCC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using SMARCC1 Polyclonal antibody (17722-1-AP)

    WB analysis of rat brain using 17722-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 17722-1-AP (SMARCC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SMARCC1 Polyclonal antibody (17722-1-AP)

    IP experiment of mouse brain using 17722-1-AP

    IP result of anti-SMARCC1 (IP:17722-1-AP, 4ug; Detection:17722-1-AP 1:600) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SMARCC1 Polyclonal antibody (17722-1-AP)

    IHC staining of human kidney using 17722-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SMARCC1 Polyclonal antibody (17722-1-AP)

    IHC staining of human kidney using 17722-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using SMARCC1 Polyclonal antibody (17722-1-AP)

    IHC staining of human appendicitis using 17722-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17722-1-AP (SMARCC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using SMARCC1 Polyclonal antibody (17722-1-AP)

    IHC staining of human malignant melanoma using 17722-1-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 17722-1-AP (SMARCC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tissue using SMARCC1 Polyclonal antibody (17722-1-AP)

    IHC staining of human ovary using 17722-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using SMARCC1 Polyclonal antibody (17722-1-AP)

    IHC staining of human ovary using 17722-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using SMARCC1 Polyclonal antibody (17722-1-AP)

    IHC staining of human placenta using 17722-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using SMARCC1 Polyclonal antibody (17722-1-AP)

    IHC staining of human placenta using 17722-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using SMARCC1 Polyclonal antibody (17722-1-AP)

    IHC staining of human testis using 17722-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using SMARCC1 Polyclonal antibody (17722-1-AP)

    IHC staining of human testis using 17722-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using SMARCC1 Polyclonal antibody (17722-1-AP)

    IHC staining of human brain using 17722-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using SMARCC1 Polyclonal antibody (17722-1-AP)

    IHC staining of human brain using 17722-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inK-562 cells, Jurkat cells, rat brain tissue, rat testis tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman malignant melanoma tissue, human appendicitis tissue, human brain tissue, human kidney tissue, human ovary tissue, human placenta tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17722-1-AP targets SMARCC1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMARCC1 fusion protein Ag12109 相同性解析による交差性が予測される生物種
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1
Calculated molecular weight 1105 aa, 123 kDa
Observed molecular weight 155-160 kDa
GenBank accession numberBC050564
Gene symbol SMARCC1
Gene ID (NCBI) 6599
RRIDAB_2191987
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMARCC1, also named as SWI/SNF complex 155 kDa subunit or BAF155, is a 1105 amino acid protein, which belongs to the SMARCC family. SMARCC1 is expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas. SMARCC1 is Involved in transcriptional activation and repression of select genes by chromatin remodeling. SMARCC1 may stimulate the ATPase activity of the catalytic subunit of the complex. SMARCC1 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). The calculated molecular weight of TCEA1 is 123 kDa, but modified SMARCC1 is about 155-160 kDa. (PMID: 24025402)

Protocols

Product Specific Protocols
WB protocol for SMARCC1 antibody 17722-1-APDownload protocol
IHC protocol for SMARCC1 antibody 17722-1-APDownload protocol
IP protocol for SMARCC1 antibody 17722-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Cancer Discov

A role for SMARCB1 in synovial sarcomagenesis reveals that SS18-SSX induces canonical BAF destruction.

Authors - Jinxiu Li
View Article
mouseCoIP

Nucleic Acids Res

HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription.

Authors - Xu Zhu
View Article

Mol Cell Biol

Growth Factor Independence 1B-Mediated Transcriptional Repression and Lineage Allocation Require Lysine-Specific Demethylase 1-Dependent Recruitment of the BHC Complex.

Authors - David McClellan
View Article