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Validation Data Gallery

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  • Western Blot (WB) analysis of RAW264.7 using SMARCD2 Polyclonal antibody (11156-1-AP)

    WB analysis of RAW264.7 using 11156-1-AP

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 11156-1-AP (SMARCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using SMARCD2 Polyclonal antibody (11156-1-AP)

    WB analysis of mouse thymus using 11156-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11156-1-AP (SMARCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using SMARCD2 Polyclonal antibody (11156-1-AP)

    IHC staining of human cervical cancer using 11156-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11156-1-AP (SMARCD2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using SMARCD2 Polyclonal antibody (11156-1-AP)

    IHC staining of mouse spleen using 11156-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11156-1-AP (SMARCD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using SMARCD2 Polyclonal antibody (11156-1-AP)

    IHC staining of mouse spleen using 11156-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11156-1-AP (SMARCD2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat spleen tissue using SMARCD2 Polyclonal antibody (11156-1-AP)

    IHC staining of rat spleen using 11156-1-AP

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 11156-1-AP (SMARCD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat spleen tissue using SMARCD2 Polyclonal antibody (11156-1-AP)

    IHC staining of rat spleen using 11156-1-AP

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 11156-1-AP (SMARCD2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SMARCD2 Polyclonal antibody

SMARCD2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11156-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2, SMARCD 2, CRACD2, BRG1-associated factor 60B, 60 kDa BRG-1/Brm-associated factor subunit B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRAW 264.7 cells, RAW264.7 cells
Positive IHC detected inmouse spleen tissue, human cervical cancer tissue, rat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11156-1-AP targets SMARCD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMARCD2 fusion protein Ag1630 相同性解析による交差性が予測される生物種
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 2
Calculated molecular weight 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC018953
Gene Symbol SMARCD2
Gene ID (NCBI) 6603
RRIDAB_2192145
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92925
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The SWI/SNF chromatin remodelling complexes are important regulators of transcription; they consist of large multisubunit assemblies containing either Brm or Brg1 as the catalytic ATPase subunit and a variable subset of approximately 10 Brg/Brm-associated factors (BAF) [PMID:20148946]. SMARCD2 is one of BAF60 proteins, which are found in most complexes, is thought to bridge interactions between transcription factors and SWI/SNF complexes. [PMID:9427560]

Protocols

Product Specific Protocols
WB protocol for SMARCD2 antibody 11156-1-APDownload protocol
IHC protocol for SMARCD2 antibody 11156-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols