SMARCE1 Polyclonal antibody
SMARCE1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 10814-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, HEK-293 cells, HeLa cells, MCF-7 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
10814-1-AP targets SMARCE1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SMARCE1 fusion protein Ag1161 相同性解析による交差性が予測される生物種 |
| Full Name | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 |
| Calculated molecular weight | 47 kDa |
| Observed molecular weight | 50-55 kDa |
| GenBank accession number | BC007082 |
| Gene symbol | SMARCE1 |
| Gene ID (NCBI) | 6605 |
| RRID | AB_2192174 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Via antagonizing chromatin-mediated transcriptional repression, the SWI/SNF complex in S. cerevisiae and Drosophila is thought to facilitate transcriptional activation of specific genes. The BRG1/brm-associated factors, or SMARCE1, complex in mammals is functionally related to SWI/SNF and consists of 9 to 12 subunits, some of which are homologous to SWI/SNF subunits. It contains a DNA-binding HMG domain and performs multiple biological functions, including DNA-, protein- and chromatin-binding, transcription coactivating activity, chromatin modification, DNA-dependent negative regulation of transcription, regulation of transcription of the RNA polymerase II promoter. In addition, SMARCE1, as an ER (estrogen receptor) subtype-selective modulator, regulates ER mediated transcription in a ligand-dependent manner and also directly binds to AR (androgen receptor) to alter its activity. The calculated molecular weight of SMARCE1 is 47 kDa, but modified SMARCE1 is about 55 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SMARCE1 antibody 10814-1-AP | Download protocol |
| IHC protocol for SMARCE1 antibody 10814-1-AP | Download protocol |
| IP protocol for SMARCE1 antibody 10814-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
iScience WTAP boosts lipid oxidation and induces diabetic cardiac fibrosis by enhancing AR methylation |
