• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Jurkat cells using SMARCE1 Monoclonal antibody (66182-1-Ig)

    WB analysis of Jurkat using 66182-1-Ig (same clone as 66182-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66182-1-Ig (SMARCE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66182-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SMARCE1 Monoclonal antibody (66182-1-Ig)

    IHC staining of human breast cancer using 66182-1-Ig (same clone as 66182-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66182-1-Ig (SMARCE1 Antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 66182-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SMARCE1 Monoclonal antibody (66182-1-Ig)

    IHC staining of human breast cancer using 66182-1-Ig (same clone as 66182-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66182-1-Ig (SMARCE1 Antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 66182-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SMARCE1 Monoclonal antibody (66182-1-Ig)

    IF Staining of HEK-293 using 66182-1-Ig (same clone as 66182-1-PBS)

    Immunofluorescent analysis of HEK-293 cells using 66182-1-Ig (SMARCE1 antibody) at dilution of 1:50 and FITC-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66182-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SMARCE1 Monoclonal antibody (66182-1-Ig)

    IF Staining of HEK-293 using 66182-1-Ig (same clone as 66182-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SMARCE1 antibody (66182-1-Ig, Clone: 1F3D6 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66182-1-PBS in a different storage buffer formulation.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

SMARCE1 Monoclonal antibody, PBS Only

SMARCE1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66182-1-PBS
Clone No.1F3D6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

BAF57, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1, BRG1-associated factor 57, BRG1 associated factor 57, 1F3D6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66182-1-PBS targets SMARCE1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SMARCE1 fusion protein Ag1161 相同性解析による交差性が予測される生物種
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
Calculated molecular weight 47 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC007082
Gene Symbol SMARCE1
Gene ID (NCBI) 6605
RRIDAB_2881577
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ969G3
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Via antagonizing chromatin-mediated transcriptional repression, the SWI/SNF complex in S. cerevisiae and Drosophila is thought to facilitate transcriptional activation of specific genes. The BRG1/brm-associated factors, or SMARCE1, complex in mammals is functionally related to SWI/SNF and consists of 9 to 12 subunits, some of which are homologous to SWI/SNF subunits. It contains a DNA-binding HMG domain and performs multiple biological functions, including DNA-, protein- and chromatin-binding, transcription coactivating activity, chromatin modification, DNA-dependent negative regulation of transcription, regulation of transcription of the RNA polymerase II promoter. In addition, SMARCE1, as an ER (estrogen receptor) subtype-selective modulator, regulates ER mediated transcription in a ligand-dependent manner and also directly binds to AR (androgen receptor) to alter its activity. The calculated molecular weight of SMARCE1 is 47 kDa, but modified SMARCE1 is about 55 kDa.