SMC1A Polyclonal antibody

Name: SMC1A Polyclonal antibody
Brand: Proteintech
SKU: 21695-1-AP

SMC1A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 21695-1-AP

Synonyms

CDLS2, DXS423E, KIAA0178, SB1.8, SMC 1 alpha, SMC 1A, SMC protein 1A, SMC1, SMC1A, SMC1alpha, SMC1L1, SMCB

Validation Data Gallery

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AllWBIHC

WB analysis using 21695-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21695-1-AP (SMC1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21695-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21695-1-AP (SMC1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 21695-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 21695-1-AP (SMC1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 21695-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 21695-1-AP (SMC1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 21695-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21695-1-AP (SMC1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human gliomas using 21695-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 21695-1-AP (SMC1A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 21695-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 21695-1-AP (SMC1A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 21695-1-AP

Size/Concentration

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, HeLa cells, K-562 cells, NIH/3T3 cells
Positive IHC detected in	human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

21695-1-AP targets SMC1A in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SMC1A fusion protein Ag16286 相同性解析による交差性が予測される生物種
Full Name	structural maintenance of chromosomes 1A
Calculated molecular weight	1233 aa, 143 kDa
Observed molecular weight	143-150 kDa
GenBank accession number	BC112127
Gene symbol	SMC1A
Gene ID (NCBI)	8243
RRID	AB_2878904
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Structural maintenance of chromosomes protein 1A (SMC1A) is involved in chromosome cohesion during cell cycle and in DNA repair. It is a central component of cohesin complex which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

Protocols

Product Specific Protocols
WB protocol for SMC1A antibody 21695-1-AP	Download protocol
IHC protocol for SMC1A antibody 21695-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Front Immunol Interactome of the Autoimmune Risk Protein ANKRD55. Authors - Nerea Ugidos View Article
human	WB,IHC	Cancer Med PIK3R3 is upregulated in liver cancer and activates Akt signaling to control cancer growth by regulation of CDKN1C and SMC1A Authors - Weidong Lin View Article
human	WB	Front Genet In-Frame Variants in STAG3 Gene Cause Premature Ovarian Insufficiency. Authors - Wen-Juan Xiao View Article
human,mouse	IF	Clin Transl Med Sulfotransferase SULT2B1 facilitates colon cancer metastasis by promoting SCD1-mediated lipid metabolism Authors - Gang Che View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMC1A Polyclonal antibody

SMC1A Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 21695-1-AP

WB analysis of HeLa using 21695-1-AP

WB analysis of K-562 using 21695-1-AP

WB analysis of A431 using 21695-1-AP

WB analysis of NIH/3T3 using 21695-1-AP

IHC staining of human gliomas using 21695-1-AP

IHC staining of human gliomas using 21695-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Interactome of the Autoimmune Risk Protein ANKRD55.

PIK3R3 is upregulated in liver cancer and activates Akt signaling to control cancer growth by regulation of CDKN1C and SMC1A

In-Frame Variants in STAG3 Gene Cause Premature Ovarian Insufficiency.

Sulfotransferase SULT2B1 facilitates colon cancer metastasis by promoting SCD1-mediated lipid metabolism