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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMC1A Monoclonal antibody (66987-1-Ig)

    WB analysis using 66987-1-Ig (same clone as 66987-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66987-1-Ig (SMC1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66987-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SMC1A Monoclonal antibody (66987-1-Ig)

    IHC staining of human colon cancer using 66987-1-Ig (same clone as 66987-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66987-1-Ig (SMC1A antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66987-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SMC1A Monoclonal antibody (66987-1-Ig)

    IHC staining of human colon cancer using 66987-1-Ig (same clone as 66987-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66987-1-Ig (SMC1A antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66987-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using SMC1A Monoclonal antibody (66987-1-Ig)

    FC experiment of HeLa using 66987-1-Ig (same clone as 66987-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SMC1A Monoclonal antibody (66987-1-Ig, Clone:1A5D2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66987-1-PBS in a different storage buffer formulation.

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SMC1A Monoclonal antibody, PBS Only

SMC1A Monoclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 66987-1-PBS
Clone No.1A5D2

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

SMC 1A, SMC 1 alpha, Sb1.8, KIAA0178, DXS423E

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66987-1-PBS targets SMC1A in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SMC1A fusion protein Ag17257 相同性解析による交差性が予測される生物種
Full Name structural maintenance of chromosomes 1A
Calculated molecular weight 1233 aa, 143 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC112127
Gene Symbol SMC1A
Gene ID (NCBI) 8243
RRIDAB_2882304
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14683
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Structural maintenance of chromosomes protein 1A (SMC1A) is involved in chromosome cohesion during cell cycle and in DNA repair. It is a central component of cohesin complex which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.