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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMC2 Recombinant antibody

SMC2 Recombinant Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241536G3

Cat no : 84835-1-RR

Synonyms

SMC protein 2, SMC 2, hCAP E, CAPE, CAP E

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of mouse brain tissue using SMC2 Recombinant antibody (84835-1-RR)

    IHC staining of mouse brain using 84835-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84835-1-RR (SMC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SMC2 Recombinant antibody (84835-1-RR)

    IHC staining of mouse brain using 84835-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84835-1-RR (SMC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84835-1-RR is SMC2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SMC2 fusion protein Ag33471 相同性解析による交差性が予測される生物種
Full Name structural maintenance of chromosomes 2
Calculated molecular weight136 kDa
GenBank accession numberNM_001042550.1
Gene symbol SMC2
Gene ID (NCBI) 10592
RRIDAB_3672143
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMC2 is a central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases (PMID: 11136719).

Protocols

Product Specific Protocols
IHC protocol for SMC2 antibody 84835-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols