SMC3 Polyclonal antibody

Name: SMC3 Polyclonal antibody
Brand: Proteintech
SKU: 14185-1-AP

SMC3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14185-1-AP

Synonyms

Chromosome-associated polypeptide, CDLS3, BMH, Basement membrane-associated chondroitin proteoglycan, Bamacan

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of HeLa using 14185-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 14185-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 14185-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 14185-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
IHC staining of human stomach using 14185-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14185-1-AP (SMC3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 14185-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14185-1-AP (SMC3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 14185-1-AP

Size/Concentration

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, Jurkat cells, mouse brain tissue
Positive IHC detected in	human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2400
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
CoIP	See 1 publications below

Product Information

14185-1-AP targets SMC3 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SMC3 fusion protein Ag5440 相同性解析による交差性が予測される生物種
Full Name	structural maintenance of chromosomes 3
Calculated molecular weight	142 kDa
Observed molecular weight	140 kDa
GenBank accession number	BC047324
Gene symbol	SMC3
Gene ID (NCBI)	9126
RRID	AB_2192625
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for SMC3 antibody 14185-1-AP	Download protocol
IHC protocol for SMC3 antibody 14185-1-AP	Download protocol
IF protocol for SMC3 antibody 14185-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	CoIP	Int J Mol Sci APEX2-Mediated Proximity Labeling Resolves the DDIT4-Interacting Proteome. Authors - Marianna Naki View Article
human	WB	Biology (Basel) In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome. Authors - Olga Gourdomichali View Article
mouse	WB	Cell Mol Life Sci RetSat stabilizes mitotic chromosome segregation in pluripotent stem cells Authors - Wanzhi Cai View Article
human	WB	Heliyon ZNF521 promotes acute myeloid leukemogenesis by suppressing the expression and acetylation of SMC3 Authors - Rong Qin View Article

製品

アプリケーション

cGMPグレード製品

サポート

カスタム対応

プロモーション

企業情報

抗体

免疫アッセイ

サイトカイン・増殖因子

ChromoTek VHH（Nanobody®）試薬

サポートツール・試薬

ゲノミクス試薬

研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMC3 Polyclonal antibody

SMC3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of HeLa using 14185-1-AP

WB analysis of Jurkat using 14185-1-AP

WB analysis of mouse brain using 14185-1-AP

WB analysis of Jurkat using 14185-1-AP

IHC staining of human stomach using 14185-1-AP

IF Staining of HeLa using 14185-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

APEX2-Mediated Proximity Labeling Resolves the DDIT4-Interacting Proteome.

In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.

RetSat stabilizes mitotic chromosome segregation in pluripotent stem cells

ZNF521 promotes acute myeloid leukemogenesis by suppressing the expression and acetylation of SMC3