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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMC6 Polyclonal antibody (14465-1-AP)

    WB analysis using 14465-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14465-1-AP (SMC6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using SMC6 Polyclonal antibody (14465-1-AP)

    WB analysis of mouse testis using 14465-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14465-1-AP (SMC6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using SMC6 Polyclonal antibody (14465-1-AP)

    IP experiment of mouse testis using 14465-1-AP

    IP result of anti-SMC6 (IP:14465-1-AP, 4ug; Detection:14465-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

  • Immunoprecipitation (IP) experiment of HeLa cells using SMC6 Polyclonal antibody (14465-1-AP)

    IP experiment of HeLa using 14465-1-AP

    IP result of anti-SMC6 (IP:14465-1-AP, 4ug; Detection:14465-1-AP 1:4000) with HeLa cells lysate 1320 ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SMC6 Polyclonal antibody (14465-1-AP)

    IHC staining of mouse testis using 14465-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14465-1-AP (SMC6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SMC6 Polyclonal antibody

SMC6 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 14465-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Synonyms

hSMC6, SMC 6, SMC protein 6, SMC6, SMC6L1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, mouse testis tissue, NIH/3T3 cells, rat testis tissue
Positive IP detected inHeLa cells, mouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14465-1-AP targets SMC6 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMC6 fusion protein Ag5812 相同性解析による交差性が予測される生物種
Full Name structural maintenance of chromosomes 6
Calculated molecular weight 126 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC039828
Gene Symbol SMC6
Gene ID (NCBI) 79677
RRIDAB_2192792
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96SB8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMC6 (Structural maintenance of chromosomes protein 6) is core component of the SMC5-SMC6 complex that is involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs) (PMID: 26983541).

Protocols

Product Specific Protocols
WB protocol for SMC6 antibody 14465-1-APDownload protocol
IHC protocol for SMC6 antibody 14465-1-APDownload protocol
IP protocol for SMC6 antibody 14465-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Virol

PJA1 Coordinates with the SMC5/6 Complex to Restrict DNA Viruses and Episomal Genes through Interferon-independent Manner.

Authors - Wei Xu
View Article