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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMCHD1 Polyclonal antibody

SMCHD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 25589-1-AP

Synonyms

KIAA0650, SMCHD1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using SMCHD1 Polyclonal antibody (25589-1-AP)

    WB analysis of HeLa using 25589-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25589-1-AP (SMCHD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SMCHD1 Polyclonal antibody (25589-1-AP)

    WB analysis of HEK-293 using 25589-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25589-1-AP (SMCHD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using SMCHD1 Polyclonal antibody (25589-1-AP)

    IP experiment of HeLa using 25589-1-AP

    IP result of anti-SMCHD1 (IP:25589-1-AP, 4ug; Detection:25589-1-AP 1:1000) with HeLa cells lysate 1085 ug.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using SMCHD1 Polyclonal antibody (25589-1-AP)

    IHC staining of mouse skeletal muscle using 25589-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25589-1-AP (SMCHD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using SMCHD1 Polyclonal antibody (25589-1-AP)

    IHC staining of mouse skeletal muscle using 25589-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25589-1-AP (SMCHD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SMCHD1 Polyclonal antibody (25589-1-AP)

    IF Staining of HEK-293 using 25589-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 25589-1-AP (SMCHD1 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25589-1-AP targets SMCHD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMCHD1 fusion protein Ag22293 相同性解析による交差性が予測される生物種
Full Name structural maintenance of chromosomes flexible hinge domain containing 1
Calculated molecular weight 2005 aa, 226 kDa
Observed molecular weight 220 kDa
GenBank accession numberBC035774
Gene symbol SMCHD1
Gene ID (NCBI) 23347
RRIDAB_2880144
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1) is considered a non-canonical member of the SMC family owing to differences in its type of ATPase domain and in its overall linear domain architecture (PMID: 32779700). SMCHD1 is best known for its role in promoting DNA methylation and gene silencing, with apparent roles in X chromosome inactivation, imprinted gene regulation, and autosome gene cluster repression (PMID: 31668908). Variations in the SMCHD1 gene are associated with two debilitating human disorders, facioscapulohumeral muscular dystrophy (FSHD) and Bosma arhinia microphthalmia syndrome (BAMS)

Protocols

Product Specific Protocols
WB protocol for SMCHD1 antibody 25589-1-APDownload protocol
IHC protocol for SMCHD1 antibody 25589-1-APDownload protocol
IF protocol for SMCHD1 antibody 25589-1-APDownload protocol
IP protocol for SMCHD1 antibody 25589-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IP

Genome Biol

The histone modification reader ZCWPW1 promotes double-strand break repair by regulating cross-talk of histone modifications and chromatin accessibility at meiotic hotspots

Authors - Shenli Yuan
View Article